Journal ArticleDOI
Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging
TLDR
Functional photoacoustic microscopy (fPAM) is reported, which provides multiwavelength imaging of optical absorption and permits high spatial resolution beyond this depth limit with a ratio of maximum imaging depth to depth resolution greater than 100.Abstract:
Although optical absorption is strongly associated with the physiological status of biological tissue, existing high-resolution optical imaging modalities, including confocal microscopy, two-photon microscopy and optical coherence tomography, do not sense optical absorption directly. Furthermore, optical scattering prevents these methods from imaging deeper than ~1 mm below the tissue surface. Here we report functional photoacoustic microscopy (fPAM), which provides multiwavelength imaging of optical absorption and permits high spatial resolution beyond this depth limit with a ratio of maximum imaging depth to depth resolution greater than 100. Reflection mode, rather than orthogonal or transmission mode, is adopted because it is applicable to more anatomical sites than the others. fPAM is demonstrated with in vivo imaging of angiogenesis, melanoma, hemoglobin oxygen saturation (sO_2) of single vessels in animals and total hemoglobin concentration in humans.read more
Citations
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Journal ArticleDOI
Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs
Lihong V. Wang,Song Hu +1 more
TL;DR: A review of the state of the art of photoacoustic tomography for both biological and clinical studies can be found in this paper, where the authors discuss the current state-of-the-art and discuss future prospects.
Journal ArticleDOI
Imaging in the era of molecular oncology
TL;DR: Advances in experimental and clinical imaging are likely to improve how cancer is understood at a systems level and should enable doctors not only to locate tumours but also to assess the activity of the biological processes within these tumours and to provide 'on the spot' treatment.
PatentDOI
In vivo tumor targeting and spectroscopic detection with surface-enhanced Raman nanoparticle tags
TL;DR: In this article, the authors described the nanostructures, methods of preparing nanostructure, methods for detecting targets in subjects, and methods of treating diseases in subjects. An embodiment, among others, includes a metallic gold surface-enhanced Raman scattering nanoparticle, a Raman reporter and a protection structure.
Journal ArticleDOI
Biomedical photoacoustic imaging
TL;DR: The underlying physical principles of the technique, its practical implementation, and a range of clinical and preclinical applications are reviewed.
Journal ArticleDOI
Photothermal therapy and photoacoustic imaging via nanotheranostics in fighting cancer
TL;DR: It is believed that PTT and PAI having noteworthy features would become promising next-generation non-invasive cancer theranostic techniques and improve the ability to combat cancers.
References
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Optical coherence tomography
David Huang,Eric A. Swanson,Charles P. Lin,Joel S. Schuman,William G. Stinson,Warren Chang,Michael R. Hee,Thomas J. Flotte,Kenton W. Gregory,Carmen A. Puliafito,James G. Fujimoto +10 more
TL;DR: OCT as discussed by the authors uses low-coherence interferometry to produce a two-dimensional image of optical scattering from internal tissue microstructures in a way analogous to ultrasonic pulse-echo imaging.
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Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
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Angiogenesis in cancer and other diseases
Peter Carmeliet,Rakesh K. Jain +1 more
TL;DR: Pathological angiogenesis is a hallmark of cancer and various ischaemic and inflammatory diseases and integrated understanding is leading to the development of a number of exciting and bold approaches to treat cancer and other diseases, but owing to several unanswered questions, caution is needed.
Journal ArticleDOI
Optical Coherence Tomography
TL;DR: The optical coherence tomograph is a new, noninvasive technical device that can obtain cross-sectional, high-resolution images-optical coherencetomographs (OCT)-of the retina that permits an accurate evaluation of various macular and chorioretinal pathologies and the early detection of glaucomatous damage.
Journal ArticleDOI
Noninvasive, infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters
TL;DR: Observations by infrared transillumination in the exposed heart and in the brain in cephalo without surgical intervention show that oxygen sufficiency for cytochrome a,a3, function, changes in tissue blood volume, and the average hemoglobin-oxyhemoglobin equilibrium can be recorded effectively and in continuous fashion for research and clinical purposes.