Journal ArticleDOI
Fundamental aspects of sperm cryobiology: The importance of species and individual differences
TLDR
It is argued that species differences in female tract anatomy, subtle differences in sperm transport mechanisms, ability to time inseminations and deliver spermatozoa effectively are powerful determinants of fertility with cryopreserved spermatozosa.About:
This article is published in Theriogenology.The article was published on 2000-01-01. It has received 558 citations till now. The article focuses on the topics: Sperm plasma membrane & Semen cryopreservation.read more
Citations
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Journal ArticleDOI
Current status of sperm cryopreservation: why isn't it better?
TL;DR: In this article, the authors reviewed aspects of sperm cryopreservation paralleled by events of capacitation and evaluated the possible roles of sperm membrane cholesterol, reactive oxygen species, and seminal plasma as mediators of sperm function.
Journal ArticleDOI
Fundamental cryobiology of reproductive cells and tissues.
TL;DR: There is an urgent need for the development of optimum cryopreservation methods for reproductive cells and tissues from many species, and a growing interest to understand the underlying cryobiological fundamentals responsible for these low survival rates for oocytes.
Journal ArticleDOI
Potential Importance of Vitrification in Reproductive Medicine
Juergen Liebermann,Frank Nawroth,Vladimir Isachenko,Evgenia Isachenko,Gohar Rahimi,Michael J. Tucker +5 more
TL;DR: Vitrification as a cryopreservation method has had very little practical impact on human-assisted reproduction, and human preimplantation embryo vitrification is still considered to be largely experimental.
Journal ArticleDOI
Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs.
TL;DR: The present work summarizes the principles of cryoinjury and the relevance of permeating and nonpermeating cryoprotective agents and speculates with new research horizons on the preservation of boar sperm, such as vitrification and freeze-drying.
Journal ArticleDOI
Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations.
TL;DR: The membrane-protecting disaccharide trehalose confers a greater cryoprotective capacity to the base extender, when added up to 100 mOsm, which is reflected in the different sperm membranes, the motile activity and in vivo fertility.
References
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Book ChapterDOI
Sperm competition and sexual selection
TL;DR: This chapter discusses Sperm Competition in Birds, Sexual Selection in Spiders and Other Arachnids, and Reproduction, Mating Strategies and Sperm competition in Marsupials and Monotremes.
Journal ArticleDOI
Freezing of living cells: mechanisms and implications
TL;DR: A new view of the mechanism of slow freezing injury ought to facilitate the development of procedures for the preservation of complex assemblages of cells of biological, medical, and agricultural significance.
Journal ArticleDOI
Revival of Spermatozoa after Vitrification and Dehydration at Low Temperatures
TL;DR: It would appear that rapidity of freezing is less important than the avoidance of surface effects, and Revival is far better when semen is frozen in bulk than when minimal amounts in capillary tubes are used.
Journal ArticleDOI
Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function.
TL;DR: The hypothesis is advanced that cryopreserved mammalian spermatozoa are in a state resembling capacitation, which accounts for their relatively reduced longevity and their readiness to undergo egg penetration without incubation.
Journal ArticleDOI
Cryopreservation of mammalian sperm: what we ask them to survive.
TL;DR: These concepts of membrane structure and the relationship of membrane composition to water and cryoprotectant movement are reviewed and reintroduced in the context of the established and successful protocol for freezing bull sperm to illustrate the molecular responses that may be necessary to survive a freeze-thaw cycle.