Open AccessJournal Article
Genetic diversity analysis and chemical profiling of Indian Acorus calamus accessions from South and North-East India
M.N. Mythili Avadhani,C. Immanuel Selvaraj,P.E. Rajasekharan,V K Rao,H Munirajappa,Tharachand C +5 more
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TLDR
The phytochemical and cytogenetic analysis of A. calamus accessions revealed that both diploid and triploid have low concentration of β-asarone irrespective of their geographical location.Abstract:
Acorus calamus L. (Family: Acoraceae) is a well-known traditional, endangered, medicinal and aromatic plant mainly found in India and China. The plant is also widely used in industrial, pharmaceutical and food industries. In the present study, 20 different accessions of Indian A. calamus were subjected to the study of genetic diversity (RAPD), and cytogenetic and phytochemical (β-asarone) analysis. For RAPD analysis, 9 primers were chosen, which generated 107 DNA fragments. The average percentage of polymorphism was recorded to be 67.23%. The primer OPA 12 showed the highest (100%) polymorphism, whereas the lowest (38.2%) polymorphism was observed for the primer OPBB 6. The polymorphism information content (PIC) values ranged 0.44 (OPA 7) to 0.18 (OPA11), while marker index (MI) values ranged 4.74 (OPA 7) to 0.36 (OPA 11). A dendogram was constructed by UPGMA method and the robustness of the tree was confirmed by bootstrap analysis with 1000 pseudo samples. For cytogenetic analysis, the 20 A. calamus accessions were screened for their ploidy status. The accessions were found to be either diploid or triploids. The phytochemical analysis of β-asarone content was determined through by HPLC method. The β-asarone concentration varied in the range of 2.2 to 7.2 mg/100 mg. The results of present study indicated the presence of low level of polymorphism among the A. calamus accessions of South India and North-East India. The phytochemical and cytogenetic analysis revealed that both diploid and triploid have low concentration of β-asarone irrespective of their geographical location.read more
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Journal ArticleDOI
Morphological characterization of diploid and triploid Acorus calamus (Acoraceae) from southern Western Siberia, parthenocarpy in sterile plants and occurrence of aneuploidy
Dmitry D. Sokoloff,M. V. Skaptsov,Nikolay A. Vislobokov,Sergey V. Smirnov,Alexander I. Shmakov,Margarita V. Remizowa +5 more
TL;DR: It is confirmed that diploids differ from triploids in the size of air lacunae in leaves, which is determined by cell number rather than cell size in septa of aerenchyma.
Book ChapterDOI
Genetic Diversity and Variability Analysis in Sweet Flag (Acorus calamus L.)
TL;DR: Based on the variation present in the genotypes as exhibited by the estimates of various parameters, continuous selection can be adopted for crop improvement in sweet flag.
Journal Article
Chemical composition and antioxidant activity of Acorus calamus L. accessions from different altitudes of Uttarakhand Himalayas
TL;DR: It can be inferred that the herb may be a good source of bioactive compounds and can work as an antioxidant to prevent the oxidative deteriorative activity of food materials beside generation of database for its scientific and judicious in-situ exploitation.
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TL;DR: The use of controlled environments can overcome cultivation difficulties and could be a means to manipulate phenotypic variation in bioactive compounds and toxins as mentioned in this paper, and molecular marker assisted selection will be used increasingly.
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The advantages of clonal integration under different ecological conditions: a community-wide test
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