Genetic instability of sugarcane plants derived from meristem cultures
Maria Imaculada Zucchi,Hideto Arizono,Vicente Alberto Morais,Maria Helena Pelegrinelli Fungaro,Maria Lucia Carneiro Vieira +4 more
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TLDR
The analysis of plants of two sugarcane varieties cultivated in vitro from meristems showed that variety RB83-5486 was more unstable than variety SP80-185, and there was no direct association with the stage of subcultivation.Abstract:
The RADP (Random amplified polymorphic DNA) technique was used to detect tissue-culture-induced variations in sugarcane. Plants of the Brazilian variety RB83-5486 propagated via rhizomes and via meristem cultures were studied. The polymorphism rate for 98 RAPD loci was 6.93% when the plants derived from meristems. Besides, in order to evaluate the influence of the number of subcultures on the generation of somaclonal variation, field-grown RB83-5486 plants derived from 10 meristems were studied after five subcultivations. Although different rates of polymorphism were observed, there was no direct association with the stage of subcultivation. The analysis of plants of two sugarcane varieties cultivated in vitro from meristems showed that variety RB83-5486 was more unstable than variety SP80-185.read more
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Genetic stability of cryopreserved shoot tips of Rubus germplasm
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Applications of in vitro culture systems for commercial sugarcane production and improvement
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Agronomic performance and genetic characterization of sugarcane transformed for resistance to sugarcane yellow leaf virus
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References
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A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
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Rapid isolation of high molecular weight plant DNA
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Journal ArticleDOI
Somaclonal variation — a novel source of variability from cell cultures for plant improvement
Philip J. Larkin,W. R. Scowcroft +1 more
TL;DR: It is argued that this variation in plant cell culture itself generates genetic variability (somaclonal variation) that may be employed to enhance the exchange required in sexual hybrids for the introgression of desirable alien genes into a crop species.
Journal ArticleDOI
Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues
TL;DR: Yields ranged from 0.3–200 nanograms of DNA per milligram of tissue, and in no case tested was inhibition observed for restriction enzymes BamHI or EcoRI.
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