Journal ArticleDOI
Genetically Engineered Resistance to Dengue-2 Virus Transmission in Mosquitoes
Ken E. Olson,S. Higgs,Patrick J. Gaines,A.M. Powers,B. S. Davis,K. I. Kamrud,J. O. Carlson,Carol D. Blair,Barry J. Beaty +8 more
TLDR
A recombinant Sindbis virus was used to transduce female Aedes aegypti with a 567-base antisense RNA targeted to the premembrane coding region of dengue type 2 (DEN-2) virus, and the transduced mosquitoes were unable to support replication of DEN-2 virus in their salivary glands and therefore were not able to transmit the virus.Abstract:
The control of arthropod-borne virus diseases such as dengue may ultimately require the genetic manipulation of mosquito vectors to disrupt virus transmission to human populations. To reduce the ability of mosquitoes to transmit dengue viruses, a recombinant Sindbis virus was used to transduce female Aedes aegypti with a 567-base antisense RNA targeted to the premembrane coding region of dengue type 2 (DEN-2) virus. The transduced mosquitoes were unable to support replication of DEN-2 virus in their salivary glands and therefore were not able to transmit the virus.read more
Citations
More filters
Journal ArticleDOI
The gut microbiota of insects - diversity in structure and function.
Philipp Engel,Nancy A. Moran +1 more
TL;DR: Gut bacteria of other insects have also been shown to contribute to nutrition, protection from parasites and pathogens, modulation of immune responses, and communication, and the extent of these roles is still unclear and awaits further studies.
Journal ArticleDOI
The Endosymbiotic Bacterium Wolbachia Induces Resistance to Dengue Virus in Aedes aegypti
TL;DR: Wolbachia inhibits viral replication and dissemination in the main dengue vector, Aedes aegypti, and is associated with an elevated basal immunity and increased longevity in the mosquitoes, underscoring the potential usefulness of Wolbachia-based control strategies for population replacement.
Journal ArticleDOI
Gene drive systems for insect disease vectors
Steven P. Sinkins,Fred Gould +1 more
TL;DR: The potential of natural gene drive systems for spreading genes that can block the transmission of insect-borne pathogens are explored and the artificial constructs that could be envisaged for this purpose are discussed.
Journal ArticleDOI
Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti
Alexander W. E. Franz,Irma Sanchez-Vargas,Zach N. Adelman,Carol D. Blair,Barry J. Beaty,Anthony A. James,Ken E. Olson +6 more
TL;DR: Engineering of transgenic A. aegypti that show a high level of resistance against DENV-2 provides a powerful tool for developing population replacement strategies to control transmission of dengue viruses.
Journal ArticleDOI
Alphavirus-based expression vectors : strategies and applications
Ilya Frolov,Thomas A. Hoffman,Béla M. Prágai,Sergey A. Dryga,Henry V. Huang,Sondra Schlesinger,Charles M. Rice +6 more
TL;DR: Alphaviruses are positive-strand RNA viruses that can mediate efficient cytoplasmic gene expression in insect and vertebrate cells that have been engineered for high-level expression of heterologous RNAs and proteins through recombinant DNA technology.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
Journal ArticleDOI
Beitrag zur kollektiven behandlung pharmakologischer reihenversuche
TL;DR: In this paper, ein Verfahren zur kollektiven behandlung von Reihenversuchen angegeben, das gestattet, bei kleinerem Umfang des Kollektivs (Reihenversuch mit vier bis funf Gruppen zu je sechs Tieren) and bei starker Streuung zu einem zahlenmasigen Ausdruck des Versuchsergebnisses zu gelangen.
Journal ArticleDOI
Flavivirus Genome Organization, Expression, and Replication
Journal ArticleDOI
Pathogenesis of dengue: challenges to molecular biology
TL;DR: This work has identified a severe syndrome, dengue hemorrhagic fever/dengue shock syndrome, in Southeast Asian children, which recently has also been identified in children infected with the virus in Puerto Rico.