The Endosymbiotic Bacterium Wolbachia Induces Resistance to Dengue Virus in Aedes aegypti
TLDR
Wolbachia inhibits viral replication and dissemination in the main dengue vector, Aedes aegypti, and is associated with an elevated basal immunity and increased longevity in the mosquitoes, underscoring the potential usefulness of Wolbachia-based control strategies for population replacement.Abstract:
Genetic strategies that reduce or block pathogen transmission by mosquitoes have been proposed as a means of augmenting current control measures to reduce the growing burden of vector-borne diseases. The endosymbiotic bacterium Wolbachia has long been promoted as a potential vehicle for introducing disease-resistance genes into mosquitoes, thereby making them refractory to the human pathogens they transmit. Given the large overlap in tissue distribution and intracellular localization between Wolbachia and dengue virus in mosquitoes, we conducted experiments to characterize their interactions. Our results show that Wolbachia inhibits viral replication and dissemination in the main dengue vector, Aedes aegypti. Moreover, the virus transmission potential of Wolbachia-infected Ae. aegypti was significantly diminished when compared to wild-type mosquitoes that did not harbor Wolbachia. At 14 days post-infection, Wolbachia completely blocked dengue transmission in at least 37.5% of Ae. aegypti mosquitoes. We also observed that this Wolbachia-mediated viral interference was associated with an elevated basal immunity and increased longevity in the mosquitoes. These results underscore the potential usefulness of Wolbachia-based control strategies for population replacement.read more
Citations
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Journal ArticleDOI
Successful establishment of Wolbachia in Aedes populations to suppress dengue transmission
Ary A. Hoffmann,Brian L. Montgomery,Jean Popovici,Jean Popovici,Inaki Iturbe-Ormaetxe,Inaki Iturbe-Ormaetxe,Petrina H. Johnson,F Muzzi,Melinda Greenfield,M Durkan,Yi San Leong,Yi Dong,Yi Dong,Helen Cook,Jason K. Axford,Ashley G. Callahan,Nichola Kenny,Nichola Kenny,C Omodei,Elizabeth A. McGraw,Elizabeth A. McGraw,Peter A. Ryan,Peter A. Ryan,Peter A. Ryan,Scott A. Ritchie,Michael Turelli,Scott Leslie O'Neill,Scott Leslie O'Neill +27 more
TL;DR: This work describes how the wMel Wolbachia infection, introduced into the dengue vector Aedes aegypti from Drosophila melanogaster, successfully invaded two natural A. aagypti populations in Australia, reaching near-fixation in a few months following releases of wMel-infected A.A. ae Egyptian adults.
Journal ArticleDOI
The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations.
Thomas Walker,Petrina H. Johnson,Luciano Andrade Moreira,Luciano Andrade Moreira,Inaki Iturbe-Ormaetxe,Francesca D. Frentiu,Conor J. McMeniman,Conor J. McMeniman,Yi San Leong,Y. Dong,Jason K. Axford,Peter Kriesner,Alun L. Lloyd,Alun L. Lloyd,Scott A. Ritchie,Scott Leslie O'Neill,Scott Leslie O'Neill,Ary A. Hoffmann +17 more
TL;DR: The successful transinfection of A. aegypti with the avirulent wMel strain of Wolbachia, which induces the reproductive phenotype cytoplasmic incompatibility with minimal apparent fitness costs and high maternal transmission, providing optimal phenotypic effects for invasion is described.
Journal ArticleDOI
Wolbachia induces reactive oxygen species (ROS)-dependent activation of the Toll pathway to control dengue virus in the mosquito Aedes aegypti
TL;DR: The results indicate that a symbiotic bacterium can manipulate the host defense system to facilitate its own persistent infection, resulting in a compromise of the mosquito's ability to host human pathogens.
Journal ArticleDOI
Wolbachia Invades Anopheles stephensi Populations and Induces Refractoriness to Plasmodium Infection
Guowu Bian,Deepak Joshi,Yuemei Dong,Peng Lu,Guoli Zhou,Xiaoling Pan,Yao Xu,George Dimopoulos,Zhiyong Xi,Zhiyong Xi +9 more
TL;DR: In this paper, the establishment of a stable Wolbachia infection in an important malaria vector, Anopheles stephensi, was reported, and the infection exhibited both perfect maternal transmission and the ability to induce high levels of cytoplasmic incompatibility.
Journal ArticleDOI
Wolbachia and the biological control of mosquito-borne disease
TL;DR: Wolbachia‐infected Aedes aegypti mosquitoes have been released in areas of Australia in which outbreaks of dengue fever occur, as a prelude to the application of this technology in d Dengue‐endemic areas of south‐east Asia.
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Luciano Andrade Moreira,Inaki Iturbe-Ormaetxe,Jason A. L. Jeffery,Guangjin Lu,Alyssa T. Pyke,Lauren M. Hedges,Bruno Coelho Rocha,Sonja Hall-Mendelin,Andrew Day,Markus Riegler,Leon E. Hugo,Karyn N. Johnson,Brian H. Kay,Elizabeth A. McGraw,Andrew F. van den Hurk,Andrew F. van den Hurk,Peter A. Ryan,Scott Leslie O'Neill +17 more
TL;DR: It is suggested that this Wolbachia-mediated pathogen interference may work synergistically with the life-shortening strategy proposed previously to provide a powerful approach for the control of insect transmitted diseases.
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