Hepatic Monooxygenase Activities in Subjects With a Genetic Defect in Drug Oxidation
Peter J. Meier,Peter J. Meier,Heinz Mueller,Heinz Mueller,Bernhard Dick,Bernhard Dick,Urs Meyer,Urs Meyer +7 more
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TLDR
It is indicated that genetically defective in vivo metabolism of debrisoquine is caused by a deficiency of a monooxygenase reaction in liver microsomes, and direct measurement of the debrisoquines oxidation deficiency may allow the identification of heterozygous carriers of the defect.About:
This article is published in Gastroenterology.The article was published on 1983-09-01 and is currently open access. It has received 117 citations till now. The article focuses on the topics: Debrisoquine.read more
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Organic anion-transporting polypeptide B (OATP-B) and its functional comparison with three other OATPs of human liver
Gerd A. Kullak-Ublick,Manfred G. Ismair,Bruno Stieger,Lukas Landmann,Robert Huber,Flavia Pizzagalli,Karin Fattinger,Peter J. Meier,Bruno Hagenbuch +8 more
TL;DR: OATP-B is the third bromosulphophthalein uptake system localized at the basolateral membrane of human hepatocytes and is the first to be characterized with respect to tissue distribution and hepatocellular localization.
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Fluvoxamine is a potent inhibitor of cytochrome P4501A2
TL;DR: The present study demonstrates that fluvoxamine is a very potent inhibitor of the high-affinity O-deethylation of phenacetin, which is catalysed by cytochrome P4501A2 (CYP1A2), in microsomes from three human livers.
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Pharmacogenetics: five decades of therapeutic lessons from genetic diversity
TL;DR: Knowing the gene variants that cause differences among patients has the potential to allow 'personalized' drug therapy and to avoid therapeutic failure and serious side effects.
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Overview of enzymes of drug metabolism
TL;DR: Biotransformation means that a lipid-soluble xenobiotic or endobiotic compound is enzymatically transformed into polar, water- soluble, and excretable metabolites.
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High-performance liquid chromatographic assays for bufuralol 1′-hydroxylase, debrisoquine 4-hydroxylase, and dextromethorphan O-demethylase in microsomes and purified cytochrome P-450 isozymes of human liver
TL;DR: Assays for the in vitro metabolism of (+)- and (-)-bufuralol, debrisoquine, and dextromethorphan in human liver microsomes and reconstituted purified cytochrome P-450 isozymes indicate the involvement of the same enzyme in the metabolism of all three substrates investigated.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
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Substrate-inducible Microsomal Aryl Hydroxylase in Mammalian Cell Culture: I. Assay and properties of induced enzyme
TL;DR: The spectrophotofluorometric determination of hydroxylated benzo[a]pyrene products is sufficiently sensitive to detect 10-12 mole per ml and has great utility in measuring the hydroxymatic activity of cells grown in culture.
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Polymorphic hydroxylation of debrisoquine in man
TL;DR: Family studies supported the view that alicyclic 4-hydroxylation of debrisoquine is controlled by a single autosomal gene and that a defect in this metabolic step is caused by a recessive allele.