Journal ArticleDOI
High-cell-density cultivation of microorganisms.
D Riesenberg,R Guthke +1 more
TLDR
Development in the application of multivariate statistical control, artificial neural networks, fuzzy control and knowledge-based supervision (expert systems) are summarized for high-cell-density cultivation, with emphasis on robust fermentation control.Abstract:
High-cell-density cultivation (HCDC) is required to improve microbial biomass and product formation substantially. An overview of HCDC is given for microorganisms including bacteria, archae and eukarya (yeasts). Problems encountered by HCDC and their possible solutions are discussed. Improvements of strains, different types of bioreactors and cultivation strategies for successful HCDC are described. Stirred-tank reactors with and without cell retention, a dialysis-membrane reactor, a gas-lift reactor and a membrane cyclone reactor used for HCDC are outlined. Recently modified traditional feeding strategies and new ones are included, in particular those for unlimited growth to very dense cultures. Emphasis is placed on robust fermentation control because of the growing industrial interest in this field. Therefore, developments in the application of multivariate statistical control, artificial neural networks, fuzzy control and knowledge-based supervision (expert systems) are summarized. Recent advances using Escherichia coli--the pioneer organism for HCDC--are outlined.read more
Citations
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Journal ArticleDOI
10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis
Igor Mierau,Michiel Kleerebezem +1 more
TL;DR: An extensive overview is provided of the different applications in lactococci and other Gram-positive bacteria: over-expression of homologous and heterologous genes for functional studies and to obtain large quantities of specific gene products, metabolic engineering, and large-scale applications.
Journal ArticleDOI
Growing E. coli to high cell density--a historical perspective on method development.
Joseph Shiloach,Rephael Fass +1 more
TL;DR: B batch, fed batch and dialysis fermentation techniques are developed, together with optimization of media composition and the application of molecular biology methods, that made it possible to grow E. coli to cell densities of up to 190 g/l (dry weight), while avoiding media precipitation and preventing acetate accumulation.
Journal ArticleDOI
Engineering xylose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production
TL;DR: In this article, an oleaginous bacterium, Rhodococcus opacus PD630, accumulates large amounts of triacylglycerols (TAGs), which can be processed into advanced liquid fuels.
Journal ArticleDOI
Ectoines in cell stress protection: uses and biotechnological production.
José M. Pastor,Manuel Salvador,Montserrat Argandoña,Vicente Bernal,Mercedes Reina-Bueno,Laszlo N. Csonka,José L. Iborra,Carmen Vargas,Joaquín J. Nieto,Manuel Cánovas +9 more
TL;DR: The state of the art in ectoines uses and applications and industrial scale production using bacteria is summarized, emphasizing the importance of reactor design and operation strategies, together with the metabolic engineering aspects and the need for feedback between wet and in silico work to optimize bioproduction.
Journal ArticleDOI
High-cell-density fed-batch cultivation of the docosahexaenoic acid producing marine alga Crypthecodinium cohnii.
TL;DR: In this article, fed-batch cultivation was studied as an alternative fermentation strategy for DHA production in Crypthecodinium cohnii, and the results showed that a feed consisting of acetic acid (50% w/w) resulted in a higher overall volumetric productivity of DHA (r(DHA)) than a feed of 50% (w/v) glucose (38 and 14 mg/L/h, respectively).
References
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Journal ArticleDOI
Multivariate statistical monitoring of process operating performance
TL;DR: Using this method of statistical data compression a multivariate monitoring procedure analogous to the univariate Shewart Chart has been developed to efficiently monitor the performance of large processes, and to rapidly detect and identify important process changes.
Journal ArticleDOI
High cell-density culture of Escherichia coli
TL;DR: The problems encountered in HCDC of E. coli are reviewed, various solutions are discussed, and feeding strategies for HCDC are described.
Journal ArticleDOI
Simple fed-batch technique for high cell density cultivation of Escherichia coli.
TL;DR: A simple fed-batch process for high cell density cultivation of Escherichia coli TG1 was developed to maintain carbon-limited growth using a defined medium and cell concentrations of 128 and 148 g per 1 dry cell weight were obtained using glucose or glycerol as carbon source.
Book ChapterDOI
High Cell-Density Fermentation
TL;DR: The information in this chapter should enable a reader with little or no experience to perform a high-cell density fermentation of a P. pastoris expression strain to achieve high expression levels with almost any good-quality fermentor, modified to accommodate this organism.
Journal ArticleDOI
Application of macroscopic balances to the identification of gross measurement errors
TL;DR: A systematic method is presented which is capable of both detecting the presence of grossly biased measurement errors and locating the source of these errors in a bioreactor through statistical hypothesis testing.
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Growing E. coli to high cell density--a historical perspective on method development.
Joseph Shiloach,Rephael Fass +1 more