Showing papers in "Biotechnology Advances in 2005"

TL;DR: Progress made on several aspects of elicitor signal transduction leading to production of plant secondary metabolites are summarized, including the integration of multiple signaling pathways into or by transcription factors, as well as the linkage of the above signal components in eliciting network through protein phosphorylation and dephosphorylation.

TL;DR: Environmental and economic benefits that biotechnology can offer in manufacturing, monitoring and waste management are highlighted and the following benefits include: greatly reduced dependence on nonrenewable fuels and other resources; reduced potential for pollution of industrial processes and products.

TL;DR: Several strategies that can be used for recombinant protein secretion in E. coli are presented and their advantages and limitations depending on the characteristics of the target protein to be produced are discussed.

TL;DR: In this paper, the use of genetic engineering to modify plants for metal uptake, transport and sequestration may open up new avenues for enhancing efficiency of phytoremediation, which may offer an effective, environmentally nondestructive and cheap remediation method.

TL;DR: B batch, fed batch and dialysis fermentation techniques are developed, together with optimization of media composition and the application of molecular biology methods, that made it possible to grow E. coli to cell densities of up to 190 g/l (dry weight), while avoiding media precipitation and preventing acetate accumulation.

TL;DR: Novel approaches to maximise the efficiency of protoplast-to-plant systems include techniques already well established for animal and microbial cells, such as electrostimulation and exposure of protoplasts to surfactants and respiratory gas carriers, especially perfluorochemicals and hemoglobin.

TL;DR: A more comprehensive understanding of the molecular and physiological basis of P uptake, transportation and utilization is leading to formulation of strategies aimed at developing better P efficient cultivars suited for sustainable cropping with less P fertilizer inputs.

TL;DR: Understanding of the stabilizing features of thermostability mechanisms will greatly facilitate reengineering of some of the mesozymes to more stable thermozymes.

TL;DR: This review describes basic and practical aspects of oxidative folding that should be considered while designing and optimizing manufacturing of proteins using chemical synthesis, semi-synthesis and a recombinant expression.

TL;DR: The rationale for using food processing to reduce/eliminate allergenicity and limitations to using this approach are discussed.

TL;DR: The role of cysteine residues and cystine bridges for the functional aggregation of food proteins and the effect of disulphide bonding between aggregates of proteins and protein mixtures on the functional macroscopic properties of space filling networks in protein gels is discussed.

TL;DR: This work reviews the natural occurrence and structural characteristics of phospholipids, their updated knowledge on manifold biological and nutritional functions, traditional and novel physical and chemical approaches to modify phospholippids as well as their applications to obtain novel phospholIPids, and brief introduction of the efforts focusing on de novo syntheses of phosphlipids.

TL;DR: An overview of the potential bioprocessing strategies that can be applied to inhibit protease activity thereby enhancing heterologous protein production is provided.

TL;DR: The Bet v 1 family of allergens includes tree pollinosis-associated food allergens with low stability which induce the symptoms of the oral allergy syndrome.

TL;DR: The stable and site-specific modification of mammalian genomes has a variety of applications in biomedicine and biotechnology and two alternative approaches that can be employed to achieve this goal are outlined: homologous recombination (HR) or site- specific recombination.

TL;DR: Although the biochemical functions of rol genes remain poorly understood, they are useful tools for improving ornamental flowers, as their expression in transgenic plants yields many beneficial traits.

TL;DR: The prospects of producing methionine by fermentation are discussed and approaches to selecting microorganism having relaxed and complex regulatory control mechanisms for Methionine biosynthesis are examined.

TL;DR: Detailed information has come available on peptide sequences that are toxic for Coeliac Disease patients, and this information is discussed in relation to prevention of the disease and the development of safe cereals for coeliac patients.

TL;DR: A brief description of the allergenic components present in the majorAllergenic foods is given followed by a short discussion regarding their importance in industrial proteins in the light of recent food labelling legislation for allergens.

TL;DR: The unified theory provides a single engineering basis for scale up of aerobic granular sludge SBRs and demonstrates that these three major selection pressures can all be unified to one, the minimal settling velocity of bio-particles, that determines aerobic granulation in S BRs.

TL;DR: This review examines methodologies for predicting protein retention time in HIC involving elution with salt gradients and considers three-dimensional structure data of the protein and its surface hydrophobicity.

TL;DR: Transgenic barley and wheat have been created to confirm the functionality of the NADP-dependent thioredoxin h system, which can be used to improve the breadmaking quality of wheat by strengthening the dough.

TL;DR: This review summarizes the available in vitro, in vivo, and informatic methods designed to evaluate different aspects of the capacity of proteins to act as true food allergens.