Journal ArticleDOI
Simple fed-batch technique for high cell density cultivation of Escherichia coli.
TLDR
A simple fed-batch process for high cell density cultivation of Escherichia coli TG1 was developed to maintain carbon-limited growth using a defined medium and cell concentrations of 128 and 148 g per 1 dry cell weight were obtained using glucose or glycerol as carbon source.About:
This article is published in Journal of Biotechnology.The article was published on 1995-02-21. It has received 463 citations till now. The article focuses on the topics: Bioreactor.read more
Citations
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Journal ArticleDOI
High cell-density culture of Escherichia coli
TL;DR: The problems encountered in HCDC of E. coli are reviewed, various solutions are discussed, and feeding strategies for HCDC are described.
Journal ArticleDOI
Growing E. coli to high cell density--a historical perspective on method development.
Joseph Shiloach,Rephael Fass +1 more
TL;DR: B batch, fed batch and dialysis fermentation techniques are developed, together with optimization of media composition and the application of molecular biology methods, that made it possible to grow E. coli to cell densities of up to 190 g/l (dry weight), while avoiding media precipitation and preventing acetate accumulation.
Journal ArticleDOI
Overcoming acetate in Escherichia coli recombinant protein fermentations.
Mark A. Eiteman,Elliot Altman +1 more
TL;DR: Recent strategies for reducing or eliminating acetate are reviewed, including approaches that optimize the protein production process as well as those that involve modifying the host organism itself.
Journal ArticleDOI
High-level production of amorpha-4,11-diene, a precursor of the antimalarial agent artemisinin, in Escherichia coli.
Hiroko Tsuruta,Christopher J. Paddon,Diana Eng,Jacob R. Lenihan,Tizita Horning,Larry Cameron Anthony,Rika Regentin,Jay D. Keasling,Jay D. Keasling,Jay D. Keasling,Neil Stephen Renninger,Jack D. Newman +11 more
TL;DR: Production of >25 g/L amorpha-4,11-diene by fermentation followed by chemical conversion to artemisinin may allow for development of a process to provide an alternative source of art Artemisinin to be incorporated into ACTs.
Journal ArticleDOI
High-cell-density cultivation of microorganisms.
D Riesenberg,R Guthke +1 more
TL;DR: Development in the application of multivariate statistical control, artificial neural networks, fuzzy control and knowledge-based supervision (expert systems) are summarized for high-cell-density cultivation, with emphasis on robust fermentation control.
References
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Journal ArticleDOI
Improved oligonudeotide site-directed rautagenesis using M13 vectors
TL;DR: An improved method is described for the construction of mutations in M13 vectors using synthetic oligonucleotides with multiple mismatches to the template, which offers an alternative strategy for construction of multiple mutations over a short region.
Journal ArticleDOI
High cell density cultivation of Escherichia coli at controlled specific growth rate.
D. Riesenberg,V. Schulz,W. A. Knorre,H. D. Pohl,D.J. Korz,Ernst Sanders,A. Ross,W.-D. Deckwer +7 more
TL;DR: The proposed HCDC suppresses generation of inhibitory by-products and the high yield coefficients indicate the economy of the process.
Journal ArticleDOI
Recombinant Protein Expression in High Cell Density Fed-Batch Cultures of Escherichia Coli
L Yee,Harvey W. Blanch +1 more
TL;DR: This review focuses on the use of fed-batch techniques to produce recombinant products in Escherichia coli with high cell concentrations as well as high specific yields of recombinant product.
Journal ArticleDOI
High-cell-density cultivation of Escherichia coli
TL;DR: High-cell-density cultivations of Escherichia coli in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only to allow optimum recombinant protein generation.
Journal ArticleDOI
High density cultivation of biomass in fed-batch system with do-stat
TL;DR: In this article, Escherichia coli and Candida brassicae were cultivated in a fed-batch culture with a DO-stat under the condition that none of the components in the basal medium limited the growth of the microorganism.
Related Papers (5)
Growing E. coli to high cell density--a historical perspective on method development.
Joseph Shiloach,Rephael Fass +1 more