Journal ArticleDOI
High pressure liquid chromatographic determination of an O-methyl,methyl ester derivative of ochratoxin A.
TLDR
Recovery of OA as OA-Me2 from chicken kidney homogenates and human plasma was quantitative following simple extraction and cleanup procedures, reaction with diazomethane, and HPLC analysis.Abstract:
The mycotoxin ochratoxin A (OA) was derivatized to an O-methyl,methyl ester (Me2) with diazomethane and then determined by high pressure liquid chromatography (HPLC). Both OA and OA-Me2 were chromatographed by reverse phase HPLC with a mobile phase of acetonitrile-water (60 + 40). An increase in retention time of 309 s was observed with OA-Me2 which was detectable at 254 nm at levels as low as 3 ng. Recovery of OA as OA-Me2 from chicken kidney homogenates and human plasma was quantitative following simple extraction and cleanup procedures, reaction with diazomethane, and HPLC analysis. The novel method described should prove useful for measuring and confirming OA in tissues and in further studies on the biological fate of this mycotoxin.read more
Citations
More filters
BookDOI
Mycotoxins and food safety
TL;DR: In this paper, the authors discuss the importance of mycotoxins in the food supply and in the built environment, and the relevance of Mycotoxin in the Food Supply and the Built Environment.
Book ChapterDOI
Methods of Analysis for Ochratoxin A
TL;DR: The mycotoxin ochratoxin A (OTA) is produced by the fungi Aspergillus alutaceus and Penicillium verrucosum and has carcinogenic, nephrotoxic, teratogenic and immunosuppressive properties.
Dissertation
Determination of Mycotoxin Levels in Poultry Feeds
TL;DR: In this article, the authors present a list of publications of the year XVI.XIV-XV List of publications and their titles, and their citation distribution, and references.
Journal ArticleDOI
Determination of Ochratoxin A in Poultry Feed by High-Performance Liquid Chromatography with a Monolithic Column
Zahid Hussain Shar,Gul Ameer Sumbal,Syed Tufail Hussain Sherazi,Huseyin Kara,Masood Hussain,Muhammad Iqbal Bhanger +5 more
TL;DR: In this paper, a monolithic column with high-performance liquid chromatography coupled with an ultraviolet detector was used for the determination of ochratoxin A in poultry feed, and the results showed that the use of the column in conjunction with sample pre-treatment provided good results for the detection of OCHRoxin A.
Related Papers (5)
Preparation, gas chromatography and mass spectrometry of methyl and trimethylsilyl esters of indomethacin
Gas chromatography-mass spectrometry studies of hippuric acid derivatives. I. Evaluation of methods for quantitative determination hippuric acid. A search for benzoylsarcosine excretion in normal urine.
U. Langenbeck,J.E. Seegmiller +1 more