High-Resolution Temporal Profiling of Transcripts during Arabidopsis Leaf Senescence Reveals a Distinct Chronology of Processes and Regulation
Emily Breeze,Elizabeth Harrison,Stuart McHattie,Linda Karen Hughes,Richard Hickman,Claire Hill,Steven J. Kiddle,Youn-Sung Kim,Christopher A. Penfold,Dafyd J. Jenkins,Cunjin Zhang,Karl Morris,Carol E. Jenner,Stephen D. Jackson,Brian Thomas,Alex Tabrett,Roxane Legaie,Jonathan D. Moore,David L. Wild,Sascha Ott,David A. Rand,Jim Beynon,Katherine J. Denby,Andrew Mead,Vicky Buchanan-Wollaston +24 more
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TLDR
Analysis of motif enrichment, as well as comparison of transcription factor families showing altered expression over the time course, identify clear groups of TFs active at different stages of leaf development and senescence, which will underpin the development of network models to elucidate the process of Senescence.Abstract:
Leaf senescence is an essential developmental process that impacts dramatically on crop yields and involves altered regulation of thousands of genes and many metabolic and signaling pathways, resulting in major changes in the leaf. The regulation of senescence is complex, and although senescence regulatory genes have been characterized, there is little information on how these function in the global control of the process. We used microarray analysis to obtain a high-resolution time-course profile of gene expression during development of a single leaf over a 3-week period to senescence. A complex experimental design approach and a combination of methods were used to extract high-quality replicated data and to identify differentially expressed genes. The multiple time points enable the use of highly informative clustering to reveal distinct time points at which signaling and metabolic pathways change. Analysis of motif enrichment, as well as comparison of transcription factor (TF) families showing altered expression over the time course, identify clear groups of TFs active at different stages of leaf development and senescence. These data enable connection of metabolic processes, signaling pathways, and specific TF activity, which will underpin the development of network models to elucidate the process of senescence.read more
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Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany
Claus Wasternack,Bettina Hause +1 more
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NAC transcription factors in plant abiotic stress responses.
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Cytokinin Signaling Networks
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Alternative Splicing at the Intersection of Biological Timing, Development, and Stress Responses
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