High temperature crystallization of lysozyme: an example of phase transition.
Pierre Jollès,Jean Berthou +1 more
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This report is an attempt to show the importance of constant temperatures (until 5.5’ in this Note) for crystallization of enzymes.About:
This article is published in FEBS Letters.The article was published on 1972-06-01 and is currently open access. It has received 102 citations till now. The article focuses on the topics: Crystallization & Lysozyme.read more
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Book ChapterDOI
Stability of Proteins Small Globular Proteins
TL;DR: The chapter discusses the stability of proteins and presents the results obtained on small compact globular proteins, which represent one single cooperative system, and the temperature-induced changes in protein, denaturational and predenaturational changes inprotein, thermodynamics of protein unfolding, and thermodynamic properties of protein.
Journal ArticleDOI
What's new in lysozyme research? Always a model system, today as yesterday.
Pierre Jollès,Jacqueline Jollès +1 more
TL;DR: A review of the main achievements realized in the lysozyme research field since the meeting held in 1972 to commemorate the fiftieth anniversary of the discovery of this enzyme is presented in this article.
Journal ArticleDOI
Liquid-Liquid Phase Separation in Supersaturated Lysozyme Solutions and Associated Precipitate Formation/Crystallization
Martin Muschol,Franz Rosenberger +1 more
TL;DR: The phase boundaries for metastable liquid-liquid separation in supersaturated hen egg white lysozyme solutions with 3, 5, and 7% (wlv) NaCl at pH= 4.5 and protein concentrations c between 40 and 400 mg/ml were determined using cloud point determinations.
Journal ArticleDOI
Relative effectiveness of various ions on the solubility and crystal growth of lysozyme.
TL;DR: Lysozyme thiocyanate and nitrate crystals belong to the monoclinic system, whereas all the others have a tetragonal lattice, and SCN- precipitates and crystallizes lysozyme at low concentration, whereas sulfate is ineffective even at high concentrations.
Journal ArticleDOI
On the crystallization of proteins.
TL;DR: Quasi-elastic light scattering was used to monitor the size and shape distribution of small aggregates in a model system (lysozyme) during the pre-nucleation stage to predict whether crystals or an amorphous precipitate would result.
References
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Journal ArticleDOI
Direct crystallization of lysozyme from egg white and some crystalline salts of lysozyme
Gordon Alderton,H. L. Fevold +1 more
TL;DR: Crystallization was effected from acid solutions of purified material containing approximately 5 per cent salt, and it was also accomplished in the isoelectric region as a result of markedly lower solubility of the crystalline product in that region.
Journal ArticleDOI
Preliminary X-ray data for some new crystalline forms of β-lactoglobulin and hen-egg-white lysozyme
Journal ArticleDOI
Structure Of Lysozyme: A Fourier Map of the Electron Density at 6 Å Resolution obtained by X-ray Diffraction
TL;DR: Structure of Lysozyme: A Fourier Map of the Electron Density at 6 A Resolution obtained by X-ray Diffraction as mentioned in this paper. But this is not the case for the structure of the enzyme.
Journal ArticleDOI
X-ray analysis of structure of human lysozyme at 6 A resolution.
TL;DR: The tertiary structures of human and hen egg white lysozyme are very similar and a comparison of the two has provided some interesting results.
Related Papers (5)
Direct crystallization of lysozyme from egg white and some crystalline salts of lysozyme
Gordon Alderton,H. L. Fevold +1 more