Host-Pathogen Interactions: VIII. Isolation of a Pathogen-synthesized Fraction Rich in Glucan That Elicits a Defense Response in the Pathogen's Host.
TLDR
A polysaccharide from the fungal pathogen Colletotrichum lindemuthianum causes browning and phytoalexin production when applied to the cut surfaces of bean (Phaseolus vulgaris) cotyledons and hypocotyls.Abstract:
A polysaccharide from the fungal pathogen Colletotrichum lindemuthianum causes browning and phytoalexin production when applied to the cut surfaces of bean (Phaseolus vulgaris) cotyledons and hypocotyls. The application of an amount of polysaccharide equivalent to less than 100 ng of glucose will elicit this response in the bean tissues. The polysaccharide has been isolated both from culture filtrates and from the mycelial walls of the fungus. Purification of the polysaccharide involved anion and cation exchange chromatography and gel filtration. The polysaccharide has an apparent molecular weight between 1,000,000 and 5,000,000 daltons, and consists predominantly of 3- and 4-linked glucosyl residues.read more
Citations
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Journal ArticleDOI
Allelopathy—An update
TL;DR: There has been a rapid advance in knowledge of mechanisms of action of known allelopathic compounds, at increasingly more fundamental levels, and evidence is mounting that inhibition of nitrification increases as succession progresses toward the climax vegetation, at least in many vegetation types.
Journal ArticleDOI
Transcriptional activation of plant defense genes by fungal elicitor, wounding, and infection.
M A Lawton,C J Lamb +1 more
TL;DR: It is concluded that transcriptional activation of defense genes characteristically underlies induction of the corresponding defense responses and expression of disease resistance.
Journal ArticleDOI
The phytoalexin response: elicitation, signalling and control of host gene expression
TL;DR: The present review considers the process of phytoalexin induction, at the molecular level, from the fungal elicitor to the early changes in host gene expression associated with its action.
Book ChapterDOI
Activation, structure, and organization of genes involved in microbial defense in plants
TL;DR: This chapter discusses the recent progress made in the molecular genetics of activation of plant defenses in response to pathogen attack with primary consideration given to the resistance to fungal and bacterial pathogens by members of the plant family Leguminosae.
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Plant Innate Immunity: Perception of Conserved Microbial Signatures
TL;DR: In plants, coregulatory receptor kinases have been identified that not only are critical for the innate immune response but also serve an essential function in other regulatory signaling pathways.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The Structure of Plant Cell Walls: I. The Macromolecular Components of the Walls of Suspension-cultured Sycamore Cells with a Detailed Analysis of the Pectic Polysaccharides.
TL;DR: The structures of the pectic polymers (the neutral arabinan, the neutral galactan, and the acidic rhamnogalacturonan) were obtained by methylation analysis of fragments of these polymers which were released from the sycamore walls by the action of a highly purified endopolygalacturonase.
Journal ArticleDOI
A gas chromatographic method for the determination of aldose and uronic Acid constituents of plant cell wall polysaccharides.
Thomas M. Jones,Peter Albersheim +1 more
TL;DR: Galacturonic acid is found to be a major component of all the cell wall polysaccharides examined, and this technique has been used to determine the changes in composition of Red Kidney bean (Phaseolus vulgaris) hypocotyl cell walls during growth, and to compare thecell wall poly Saccharide compositions of several parts of bean plants.
Journal ArticleDOI
Preparation and Purification of Glucanase and Chitinase from Bean Leaves
TL;DR: Both glucanase and chitinase were extracted from ethylene-treated bean leaves and purified on hydroxyapatite and carboxymethyl Sephadex columns and had an isoelectric point near pH 11 and was specific for beta-1, 3-linkages.
Journal ArticleDOI
The Structure of Plant Cell Walls: II. The Hemicellulose of the Walls of Suspension-cultured Sycamore Cells.
TL;DR: The molecular structure, chemical properties, and biological function of the xyloglucan polysaccharide isolated from cell walls of suspension-cultured sycamore (Acer pseudoplatanus) cells are described and indicate that xylglucan is covalently linked to the pecticpolysaccharides and is noncovalently bound to the cellulose fibrils of the Sycamore cell wall.