Journal ArticleDOI
Human chromatin from interphase to metaphase: a scanning electron microscopic study.
TLDR
Scanning electron microscopy of DNase-treated whole-mount chromosomes might allow for more detailed analyses of deletions and translocations by chromomere number and position than is possible by light microscopy banding techniques.About:
This article is published in Experimental Cell Research.The article was published on 1974-03-15. It has received 44 citations till now. The article focuses on the topics: Chromomere & Premature chromosome condensation.read more
Citations
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Scanning electron microscopy of mammalian chromosomes from prophase to telophase.
TL;DR: It is proposed that the function of centromeric heterochromatin is to hold the chromatids together until anaphase, when they are separated by the concerted action of topoisomerase II acting on numerous similar sites provided by the repetitive nature of the satellite DNA in the heterochromaatin.
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Evolution of genome size by DNA doublings.
Arnold H. Sparrow,Anne F. Nauman +1 more
TL;DR: The data demonstrate the apparent existence of an exponential periodicity over eight orders of magnitude, leading the authors to suggest an evolutionary continuity of doublings of a basic ancestral genome (of about 300 nucleotides), these doubling being independent of both chromosome number and ploidy level.
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Scanning electron microscopy of the G-banded human karyotype
TL;DR: It is proposed that the observation of G-bands relies on LM visualization of quaternary structure by accumulation of Giemsa stain between the coils.
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Biochemical and Electron‐Microscopic Evidence that the Subunit Structure of Chinese‐Hamster‐Ovary Interphase Chromatin Is Conserved in Mitotic Chromosomes
TL;DR: Biochemical and electron microscopic studies demonstrate that the subunit structure of Chinese hamster ovary cell interphase chromatin is conserved in mititic chromosomes.
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Gene control in eukaryotes and the c-value paradox "excess" DNA as an impediment to transcription of coding sequences.
TL;DR: Ways in which control of gene activity may lead to the observed high DNA content per haploid eukaryote genome are examined and it is proposed that deoxyribonucleoprotein (DNP) acts as a barrier to transcription at two distinct structural levels.
References
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Techniques for the preservaation of three-dimensional structure in preparing specimens for the electron microscope†
TL;DR: In this paper, a method of sample preparation that reduces distortion and maintains 3D structure is described, where a droplet is placed on a formvar-coated screen and fix with osmic acid vapor.
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New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
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The 24 fluorescence patterns of the human metaphase chromosomes - distinguishing characters and variability.
TL;DR: Apart from certain minor but well defined chromosome regions with especially strong fluorescence, which are subject to certain individual variations, the fluorescence patterns were shown to be quite stable and reproducible.
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Analysis of Human Metaphase Chromosome Set by Aid of DNA-Binding Fluorescent Agents
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Chromosome-sized DNA molecules from Drosophila
Ruth Kavenoff,Bruno H. Zimm +1 more
TL;DR: Ch Chromosome-sized DNA molecules were obtained almost quantitatively from unsynchronized cultured cells, suggesting that the size of the chromosomal DNA is conserved throughout much of the cell cycle.