Journal ArticleDOI
Human sarcoma cells in culture: Identification by colony-forming ability on monolayers of normal cells☆
Reads0
Chats0
TLDR
The system provides a method of selecting cells that have decreased contact inhibition of cell division and may be used to select tumor cells from a population containing a large excess of normal cells.About:
This article is published in Experimental Cell Research.The article was published on 1970-07-01. It has received 77 citations till now. The article focuses on the topics: Contact inhibition & Cell culture.read more
Citations
More filters
Journal ArticleDOI
Characterization of a newly derived human sarcoma cell line (ht-1080)
TL;DR: A tumor cell line was derived from the fibrosarcoma of a 35‐year‐old Caucasian man who died without having received chemotherapy or radiotherapy, and an aberrant karyology with marker chromosomes was present.
Journal ArticleDOI
Establishment of a continuous tumor‐cell line (PANC‐1) from a human carcinoma of the exocrine pancreas
TL;DR: An epithelioid cell line, started from a human pancreatic carcinoma of ductal cell origin, has been maintained in culture for over 2 years and has been subcultured more than 40 times and has a modal number of 63 with three distinct marker chromosomes and a small ring chromosome.
Journal ArticleDOI
Cellular genes analogous to retroviral onc genes are transcribed in human tumour cells
Alessandra Eva,Keith C. Robbins,P R Andersen,A. Srinivasan,Steven R. Tronick,E. Premkumar Reddy,Nelson Ellmore,Angela T. Galen,James A. Lautenberger,Takis S. Papas,Eric H. Westin,Flossie Wong-Staal,Robert C. Gallo,Stuart A. Aaronson +13 more
TL;DR: Polyadenylated RNAs of certain human tumour cell lines are shown to contain transcripts related to the cell-derived transforming onc genes of molecularly cloned primate, murine or avian transforming retrovirus genomes.
Journal ArticleDOI
Differential expression of the amv gene in human hematopoietic cells.
Eric H. Westin,Robert C. Gallo,Suresh K. Arya,Alessandra Eva,L M Souza,M A Baluda,Stuart A. Aaronson,Flossie Wong-Staal +7 more
TL;DR: In this article, total cellular RNAs from a variety of fresh and culture-derived human hematopoietic neoplastic cell types at various stages of differentiation were enriched for poly(A)-containing sequences, fractionated by gel electrophoresis, and hybridized to a cloned DNA probe containing the transforming sequences (v-amv) of avian myeloblastosis virus (AMV), a virus known to cause myeloid leukemias in chickens.
Journal ArticleDOI
New chromosome techniques in the study of human neoplasia.
TL;DR: Amethopterin synchronization of bone marrow and lymph node cells makes it possible to obtain well banded and elongated metaphases and prometaphases in the majority of patients with leukemia and lymphoma.
References
More filters
Journal ArticleDOI
The limited in vitro lifetime of human diploid cell strains
TL;DR: The survival curves obtained with human diploid cell strains are comparable to “multiple-hit” or “ multiple-target” curves obtain with other biological systems where an initial threshold dose is required before an exponential form of the curve is established.
Journal ArticleDOI
Agar suspension culture for the selective assay of cells transformed by polyoma virus.
Ian Macpherson,Luc Montagnier +1 more
Journal ArticleDOI
Basis for the acquisition of malignant potential by mouse cells cultivated in vitro.
TL;DR: The tissue-culture property that correlates best with tumorigenicity is the loss of contact inhibition of cell division in mouse embryo lines maintained in culture under conditions that minimize cell-cell contact.
Journal ArticleDOI
Two established in vitro cell lines from human mesenchymal tumours
Jan Ponten,Eero Saksela +1 more
TL;DR: The described cell lines are the first derived from human mesenchymal tumours where the availability of a control fibroblast strain will provide us with opportunities to compare autologous normal and neoplastic human meschymal cells under in vitro conditions.
Journal ArticleDOI
Susceptibility of human diploid fibroblast strains to transformation by SV40 virus.
TL;DR: A quantitative system has been developed for the study of transformation of human diploid fibroblasts in culture by two oncogenic viruses, SV40 and the E46 strain of adeno 7-SV40 "hybrid" virus.