Journal ArticleDOI
Hydrogen peroxide production and the release of carbon dioxide during glycollate oxidation in leaf peroxisomes.
Bernard Grodzinski,V. S. Butt +1 more
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The rate at which H2O2 becomes available during glycollate oxidation for further oxidation reactions, especially that of glyoxylate to formate and CO2, in peroxisomes from spinach-beet leaves has been determined by measuring O2 uptake in the presence and absence of added catalase.Abstract:
The rate at which H2O2 becomes available during glycollate oxidation for further oxidation reactions, especially that of glyoxylate to formate and CO2, in peroxisomes from spinach-beet (Beta vulgaris L., var. vulgaris) leaves has been determined by measuring O2 uptake in the presence and absence of added catalase. The rates observed under air and pure O2 were sufficient to account for the 14CO2 released from [l-14C]glycollate under these conditions; the two reactions showed similar characteristics. In the course of the reaction, a fall in catalase activity was observed concomitant with an increase in 14CO2 release. There is no evidence that catalase was disproportionately lost from the peroxisomes during isolation, and it is argued that the CO2 release observed contributes to the photorespiratory CO2 loss in intact leaves.read more
Citations
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Photorespiration and photoinhibition: Some implications for the energetics of photosynthesis
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Microbodies in Higher Plants
TL;DR: Glyoxysome Biogenesis of Leaf Peroxisomes and Hormonal Influences: Foundations of Microbody Transition in Fatty Cotyledons.
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The regulation and control of photorespiration
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Organic Acids: The Pools of Fixed Carbon Involved in Redox Regulation and Energy Balance in Higher Plants
TL;DR: Organic acids play a role in plants in providing redox equilibrium, supporting ionic gradients on membranes, and acidification of the extracellular medium.
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Malate dehydrogenase isoenzymes: cellular locations and role in the flow of metabolites between the cytoplasm and cell organelles.
TL;DR: The review endeavours to give up-to-date information on the biogenesis and intracellular targeting of malate dehydrogenase isoenzymes as well as enzymes cooperating with them in the flow of metabolites of a given pathway and organelle.
References
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Journal ArticleDOI
Copper enzymes in isolated chloroplasts. polyphenoloxidase in beta vulgaris
TL;DR: Evidence that a copper enzyme, polyphenoloxidase (otherwise known as tyrosinase or catecholase), is localized in the chloroplasts of spinach beet (chard), Beta vu?garis is presented.
Book
Methods of Enzymatic Analysis
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.
Journal ArticleDOI
The cellular production of hydrogen peroxide
TL;DR: The cytochrome c peroxidase assay is suitable for use with subcellular fractions from tissue homogenates as well as with pure enzyme systems to measure H(2)O( 2) generation and can be made on the basis of the rates with the isolated fractions under physiological conditions.