Journal ArticleDOI
Hydrophilic-Interaction Chromatography with UV Detection for Analysis of Strychnine and Brucine in the Crude Seeds of Strychnos nux-vomica and Their Processed Products
TLDR
An easy and rapid method based on hydrophilic-interaction chromatography has been developed and validated for measurement of two major alkaloids, strychnine and brucine, in Strychnos nux-vomica plant seeds and preparations as mentioned in this paper.Abstract:
An easy and rapid method based on hydrophilic-interaction chromatography has been developed and validated for measurement of two major alkaloids, strychnine and brucine, in Strychnos nux-vomica plant seeds and preparations. The seeds were processed in different ways. Separation was performed at ambient temperature on a carbohydrate column with acetonitrile–water 90:10 (v/v) as mobile phase at a flow rate of 1.0 mL min−1. Detection was at 254 nm. Calibration plots were linear over the range 0.1–200 μg mL−1 for both compounds. Average recoveries of strychnine and brucine from spiked samples were 99.9 and 98.5%, respectively.read more
Citations
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Journal ArticleDOI
Seeing the unseen of Chinese herbal medicine processing (Paozhi): advances in new perspectives.
Xu Wu,Shengpeng Wang,Junrong Lu,Junrong Lu,Yong Jing,Mingxing Li,Jiliang Cao,Baolin Bian,Chang-jiang Hu +8 more
TL;DR: The role of processing in TCM and several typical methods of processing are introduced and the up-to-date efforts on the mechanistic study of CHM processing are summarized.
Journal ArticleDOI
A review on medicinal uses, analytical techniques and pharmacological activities of Strychnos nux-vomica Linn.: A concise report.
TL;DR: Information about Strychnos nux-vomica, compiled in the present review article could be useful to the researchers for the scientific validation of its traditional claim in the future.
References
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Journal ArticleDOI
Hydrophilic interaction chromatography.
Petrus Hemström,Knut Irgum +1 more
TL;DR: The review attempts to summarize the ongoing discussion on the separation mechanism and gives an overview of the stationary phases used and the applications addressed with this separation mode in LC.
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Is hydrophilic interaction chromatography with silica columns a viable alternative to reversed-phase liquid chromatography for the analysis of ionisable compounds?
TL;DR: The separation of acidic, neutral and particularly basic solutes was investigated using a bare silica column, mostly under hydrophilic interaction chromatography (HILIC) conditions with water concentrations >2.5% and with >70% acetonitrile (ACN).
Journal ArticleDOI
Stationary phases for hydrophilic interaction chromatography, their characterization and implementation into multidimensional chromatography concepts
TL;DR: This review attempts to present an overview of the HIL IC separation systems, possibilities for their characterization and emerging HILIC applications in 2-D off-line and on-line LC-LC separations of various samples, in combination with RP and other separation modes.
Journal ArticleDOI
Analgesic and anti-inflammatory properties of brucine and brucine N-oxide extracted from seeds of Strychnos nux-vomica.
TL;DR: The results suggest that central and peripheral mechanism are involved in the pain modulation and anti-inflammation effects of brucine and Brucine N-oxide, biochemical mechanisms of bru cine and brucines N -oxide are different even though they are similar in chemical structure.
Journal ArticleDOI
Mixed-mode ion-exchangers and their comparative chromatographic characterization in reversed-phase and hydrophilic interaction chromatography elution modes
Michael Lämmerhofer,Martin Richter,JunYan Wu,Raquel Nogueira,Wolfgang Bicker,Wolfgang Lindner +5 more
TL;DR: In this paper, a set of particulate silica-supported mixed-mode RP/weak anion-exchangers (RP/WAX) were chromatographically characterized in comparison to selected commercially available columns using two distinct isocratic elution modes, viz. an aqueous-rich RP-type elution mode (with 40% ACN and 60% buffer) as well as an organic solvent-rich hydrophilic interaction chromatography (HILIC), which showed multimodal applicability, unlike a polar embedded RP material (Synergi Fusion RP),