Identification of trypanosomes in wild animals from Southern Cameroon using the polymerase chain reaction (PCR)
TLDR
PCR was used to detect the different trypanosome species present in wild animal captured by hunters in the southern forest belt of Cameroon and found parasites pathogenic to man in 164 animals belonging to 24 different species including ungulates, rodents, pangolins, carnivores, reptiles and primates.Abstract:
One possible explanation of the maintenance of many historical foci of sleeping sickness in Central Africa could be the existence of a wild animal reservoir. In this study, PCR was used to detect the different trypanosome species present in wild animal captured by hunters in the southern forest belt of Cameroon (Bipindi). Trypanosomes were also detected by a parasitological method (Quantitative buffy coat: QBC). Parasite could not be isolated in culture medium (Kit for in vitro isolation: KIVI). Specific primers of T. brucei s.l., T. congolense forest type, T. congolense savannah type, T. vivax, T. simiae and T. b. gambiense group 1 were used to identify parasites in the blood of 164 animals belonging to 24 different species including ungulates, rodents, pangolins, carnivores, reptiles and primates. Of the 24 studied species, eight were carrying T. b. gambiense group 1. Those parasites pathogenic to man were found in monkeys (Cercocebus torquatus and Cercopithecus nictitans), in ungulates (Cephalophus dorsalis and C. monticola), in carnivores (Nandinia binotata and Genetta servalina) and in rodents (Cricetomys gambianus and Atherurus africanus). 13 species (54%) were carrying T. brucei s.l. identified as non-gambiense group 1.read more
Citations
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Spatial meta-analysis of the occurrence and distribution of tsetse-transmitted animal trypanosomiasis in Cameroon over the last 30 years
TL;DR: A systematic review of data on AAT and tsetse from 1990 to 2021 was conducted to develop a national atlas as discussed by the authors , with three pathogenic Trypanosoma species (T. vivax, T. congolense and T. brucei s.l.) most frequently identified as causing AAT.
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Activity patterns and burrowing ecology of the giant pouched rat (Cricetomys emini) in Tshuapa Province, D. R. Congo
Lem’s N. Kalemba,Clint N Morgan,Yoshinori Nakazawa,Matthew R. Mauldin,Jean Malekani,Jeffrey B. Doty +5 more
TL;DR: The present study does confirm nocturnal activity and a bimodal pattern, and suggests greater diurnal activity as compared to previous studies, but also suggests that C. emini may not change burrows as frequently as previously reported.
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Comportements humains et risque de transmission de la maladie du sommeil en zone périurbaine de Kinshasa (RDC)
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Storage time effect on blood diet for tsetse mass production in sterile insect technique.
TL;DR: There was a significant difference between the blood batches and the production parameters were better in the last three years, i.e. 2008, 2007 and 2006, and this study recommends three years to be an optimum storage time for blood diet under regional conditions.
References
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Journal Article
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The identification of Trypanosoma brucei gambiense in Liberian pigs and dogs by isoenzymes and by resistance to human plasma.
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Will the real Trypanosoma b. gambiense please stand up.
TL;DR: Wendy Gibson reviews the status of T. b.
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The isolation of Trypanosoma rhodesiense from a bushbuck.
TL;DR: If the possibility of a bleeding state after incompatible transfusion had been known, the second laparotomy would probably not have been carried out, and appropriate treatment might have been started earlier, as the patient had developed abnormal bleeding before the dextran was given.