Identification of trypanosomes in wild animals from Southern Cameroon using the polymerase chain reaction (PCR)
TLDR
PCR was used to detect the different trypanosome species present in wild animal captured by hunters in the southern forest belt of Cameroon and found parasites pathogenic to man in 164 animals belonging to 24 different species including ungulates, rodents, pangolins, carnivores, reptiles and primates.Abstract:
One possible explanation of the maintenance of many historical foci of sleeping sickness in Central Africa could be the existence of a wild animal reservoir. In this study, PCR was used to detect the different trypanosome species present in wild animal captured by hunters in the southern forest belt of Cameroon (Bipindi). Trypanosomes were also detected by a parasitological method (Quantitative buffy coat: QBC). Parasite could not be isolated in culture medium (Kit for in vitro isolation: KIVI). Specific primers of T. brucei s.l., T. congolense forest type, T. congolense savannah type, T. vivax, T. simiae and T. b. gambiense group 1 were used to identify parasites in the blood of 164 animals belonging to 24 different species including ungulates, rodents, pangolins, carnivores, reptiles and primates. Of the 24 studied species, eight were carrying T. b. gambiense group 1. Those parasites pathogenic to man were found in monkeys (Cercocebus torquatus and Cercopithecus nictitans), in ungulates (Cephalophus dorsalis and C. monticola), in carnivores (Nandinia binotata and Genetta servalina) and in rodents (Cricetomys gambianus and Atherurus africanus). 13 species (54%) were carrying T. brucei s.l. identified as non-gambiense group 1.read more
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Entomological patterns in the human African trypanosomiasis focus of Komo Mondah, Gabon.
TL;DR: Swamp mangrove would be targeted in priority during a vector control campaign for sleeping sickness in Komo Mondah because of the higher index of epidemiological risk and populations are exposed to the disease when they go to the area for taking their fishing boats.
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Prevalence of Sodalis glossinidius and different trypanosome species in Glossina palpalis palpalis caught in the Fontem sleeping sickness focus of the southern Cameroon.
TL;DR: The presence of S. glossinidius does not favor trypanosome infections in G. palpalis of the Fontem focus and statistical analyses were performed to look for an association between these microorganisms.
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Xenomonitoring of sleeping sickness transmission in Campo (Cameroon)
Pascal Grébaut,Trésor Melachio,Simplice Nyangmang,Vincent Ebo’o Eyenga,Guy-Roger Njitchouang,Elvis Ofon,Flobert Njiokou,Gustave Simo +7 more
TL;DR: Xenomonitoring provides a valuable guide/tool to determine places at higher risk for vector/human contact and to identify trypanosomes species circulating in the focus of Campo, Cameroon.
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Analysis of the domestic animal reservoir at a micro-geographical scale, the Fontem sleeping sickness focus (South-West Cameroon).
Guy R. Njitchouang,Flobert Njiokou,H. C. Nana Djeunga,P. Moundipa Fewou,Tazoacha Asonganyi,Gérard Cuny,Gustave Simo +6 more
TL;DR: The detection of T. b.
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Challenges towards the elimination of Human African Trypanosomiasis in the sleeping sickness focus of Campo in southern Cameroon
Gustave Simo,Jean Arthur Mbida Mbida,Vincent Ebo’o Eyenga,Tazoacha Asonganyi,Flobert Njiokou,Pascal Grébaut +5 more
TL;DR: The sleeping sickness focus of Campo lies along the Atlantic coast and extends along the Ntem River, which constitutes the Cameroonian and Equatorial Guinean border, and is a hypo-endemic focus with the disease prevalence varying from 0.3 to 0.86% during the last few decades.
References
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Journal Article
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Will the real Trypanosoma b. gambiense please stand up.
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