Identification of trypanosomes in wild animals from Southern Cameroon using the polymerase chain reaction (PCR)
TLDR
PCR was used to detect the different trypanosome species present in wild animal captured by hunters in the southern forest belt of Cameroon and found parasites pathogenic to man in 164 animals belonging to 24 different species including ungulates, rodents, pangolins, carnivores, reptiles and primates.Abstract:
One possible explanation of the maintenance of many historical foci of sleeping sickness in Central Africa could be the existence of a wild animal reservoir. In this study, PCR was used to detect the different trypanosome species present in wild animal captured by hunters in the southern forest belt of Cameroon (Bipindi). Trypanosomes were also detected by a parasitological method (Quantitative buffy coat: QBC). Parasite could not be isolated in culture medium (Kit for in vitro isolation: KIVI). Specific primers of T. brucei s.l., T. congolense forest type, T. congolense savannah type, T. vivax, T. simiae and T. b. gambiense group 1 were used to identify parasites in the blood of 164 animals belonging to 24 different species including ungulates, rodents, pangolins, carnivores, reptiles and primates. Of the 24 studied species, eight were carrying T. b. gambiense group 1. Those parasites pathogenic to man were found in monkeys (Cercocebus torquatus and Cercopithecus nictitans), in ungulates (Cephalophus dorsalis and C. monticola), in carnivores (Nandinia binotata and Genetta servalina) and in rodents (Cricetomys gambianus and Atherurus africanus). 13 species (54%) were carrying T. brucei s.l. identified as non-gambiense group 1.read more
Citations
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Intestinal Bacterial Communities of Trypanosome-Infected and Uninfected Glossina palpalis palpalis from Three Human African Trypanomiasis Foci in Cameroon
Franck Jacob,Tresor Tito Tanekou Melachio,Guy Roger Njitchouang,Geoffrey Gimonneau,Flobert Njiokou,Luc Abate,Richard Christen,Julie Reveillaud,Anne Geiger +8 more
TL;DR: The most important result was the evidence of the overall very large diversity of intestinal bacteria which, except for Wigglesworthia, were unevenly distributed over the sampled flies regardless of their geographic origin and their trypanosome infection status.
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Molecular evidence of a Trypanosoma brucei gambiense sylvatic cycle in the human african trypanosomiasis foci of Equatorial Guinea.
Carlos Cordon-Obras,Yasmin Fermin Rodriguez,Amalia Fernandez-Martinez,Jorge Cano,Nicolas Ndong-Mabale,Policarpo Ricardo Ncogo-Ada,Pedro Ndongo-Asumu,Pilar Aparicio,Miguel Navarro,Agustín Benito,Jean-Mathieu Bart,Jean-Mathieu Bart +11 more
TL;DR: The hypothesis that animals may play a role in the persistence of T. b.
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The composition and abundance of bacterial communities residing in the gut of Glossina palpalis palpalis captured in two sites of southern Cameroon
Jean Marc Tsagmo Ngoune,Jean Marc Tsagmo Ngoune,Julie Reveillaud,Guilhem Sempere,Flobert Njiokou,Trésor Melachio,Luc Abate,Majoline T. Tchioffo,Anne Geiger,Anne Geiger +9 more
TL;DR: A very large diversity of bacteria was identified with the discovering of species reported to secrete anti-parasitic compounds or to modulate vector competence in other insects.
Journal ArticleDOI
Molecular identification of Trypanosoma brucei gambiense in naturally infected pigs, dogs and small ruminants confirms domestic animals as potential reservoirs for sleeping sickness in Chad.
Joël Vourchakbé,Zebaze Arnol Auvaker Tiofack,Tagueu Sartrien Kante,Mbida Mpoame,Gustave Simo +4 more
TL;DR: The identification of T. b.
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Trypanosoma brucei s.l.: Microsatellite markers revealed high level of multiple genotypes in the mid-guts of wild tsetse flies of the Fontem sleeping sickness focus of Cameroon.
TL;DR: The results show that there is a wide range of trypanosome genotypes circulating in the mid-guts of wild tsetse flies from the Fontem sleeping sickness focus, which open new avenues to undertake investigations on the maturation of multiple infections observed in the t setse fly mid-Guts.
References
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Sensitive detection of trypanosomes in tsetse flies by DNA amplification.
TL;DR: The methodology should be suitable for large-scale epidemiological studies and was sensitive and specific, detecting one trypanosome unequivocally and showing no reaction with non-target trypanOSome DNA or a huge excess of host DNA.
Journal Article
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Journal Article
The identification of Trypanosoma brucei gambiense in Liberian pigs and dogs by isoenzymes and by resistance to human plasma.
TL;DR: Two stocks from pigs were found both to be resistant to human plasma and to have an isoenzyme marker, a slow alanine aminotransferase (ALAT) pattern, previously found only in Trypanosoma brucei gambiense from man, constitutes evidence that the pig is a reservoir of human trypanosomiasis in West Africa.
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Will the real Trypanosoma b. gambiense please stand up.
TL;DR: Wendy Gibson reviews the status of T. b.
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The isolation of Trypanosoma rhodesiense from a bushbuck.
TL;DR: If the possibility of a bleeding state after incompatible transfusion had been known, the second laparotomy would probably not have been carried out, and appropriate treatment might have been started earlier, as the patient had developed abnormal bleeding before the dextran was given.