Journal ArticleDOI
Immunobiosensor Chips for Detection of Escherichia coli O157:H7 Using Electrochemical Impedance Spectroscopy
TLDR
Electrochemical impedance spectroscopy and cyclic voltammetric method were employed to follow the stepwise assembly of the systems and the electronic transduction for the detection of E. coli.Abstract:
Impedance biosensor chips were developed for detection of Escherichia coli O157:H7 based on the surface immobilization of affinity-purified antibodies onto indium tin oxide (ITO) electrode chips. The immobilization of antibodies onto ITO chips was carried out using an epoxysilane monolayer to serve as a template for chemical anchoring of antibodies. The surface characteristics of chips before and after the binding reaction between the antibodies and antigens were characterized by atomic force microscopy (AFM). The patterns of the epoxysilanes monolayer, antibodies, and E. coli cells were clearly observed from the AFM images. Alkaline phosphatase as the labeled enzyme to anti-E. coli O157:H7 antibody was used to amplify the binding reaction of antibody−antigen on the chips. The biocatalyzed precipitation of 5-bromo-4-chloro-3-indolyl phosphate by alkaline phosphatase on the chips in pH 10 PBS buffer containing 0.1 M MgCl2 increased the electron-transfer resistance for a redox probe of Fe(CN)63-/4- at the e...read more
Citations
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Journal ArticleDOI
The use of electrochemical impedance spectroscopy for biosensing
F. Lisdat,D. Schäfer +1 more
TL;DR: The focus of this review is on the application of this transduction method for sensing purposes and examples of its use in combination with enzymes, antibodies, DNA and with cells will be described.
Journal ArticleDOI
Electrical/electrochemical impedance for rapid detection of foodborne pathogenic bacteria
Liju Yang,Rashid Bashir +1 more
TL;DR: This paper reviews the progress and applications of impedance microbiology for foodborne pathogenic bacteria detection, particularly the new aspects that have been added to this subject in the past few years, including the use of interdigitated microelectrodes, the development of chip-based impedance microbiologists, and theUse of equivalent circuits for analysis of the impedance systems.
Journal ArticleDOI
Interdigitated array microelectrodes based impedance biosensors for detection of bacterial cells.
Madhukar Varshney,Yanbin Li +1 more
TL;DR: This article limits its discussion to IDAM based impedance biosensors for their applications in the detection of bacterial cells to include different IDAM geometries their fabrication materials and design parameters, and types of detection techniques.
Journal ArticleDOI
Aptamer biosensor for label-free impedance spectroscopy detection of proteins based on recognition-induced switching of the surface charge.
TL;DR: The recognition of proteins by aptamer-modified electrode transducers reverses the surface charge and leads to a novel label-free impedance spectroscopy bioelectronic detection protocol based on a decrease in the electron transfer resistance.
Patent
Fluid control structures in microfluidic devices
TL;DR: In this paper, a monolithic elastomer membrane associated with an integrated pneumatic manifold allows the placement and actuation of a variety of fluid control structures, such as structures for pumping, isolating, mixing, routing, merging, splitting, preparing, and storing volumes of fluid.
References
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