Journal ArticleDOI
Improved COI barcoding primers for Southeast Asian perching birds (Aves: Passeriformes).
TLDR
Using COI sequences from complete mitochondrial genomes, a primer pair is designed that more reliably amplifies and sequences the COI barcoding region of Southeast Asian passerine birds.Abstract:
The All Birds Barcoding Initiative aims to assemble a DNA barcode database for all bird species, but the 648-bp ‘barcoding’ region of cytochrome c oxidase subunit I (COI) can be difficult to amplify in Southeast Asian perching birds (Aves: Passeriformes). Using COI sequences from complete mitochondrial genomes, we designed a primer pair that more reliably amplifies and sequences the COI barcoding region of Southeast Asian passerine birds. The 655-bp region amplified with these primers overlaps the COI region amplified with other barcoding primer pairs, enabling direct comparison of sequences with previously published DNA barcodes.read more
Citations
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DNA barcoding of Scandinavian birds reveals divergent lineages in trans-Atlantic species
Arild Johnsen,Eirik Rindal,Per G. P. Ericson,Dario Zuccon,Kevin C. R. Kerr,Mark Y. Stoeckle,Jan T. Lifjeld +6 more
TL;DR: This study demonstrates the effectiveness of COI barcodes for identifying Scandinavian birds and highlights taxa for taxonomic review.
DNA barcoding of Scandinavian birds reveals divergent lineages in trans-Atlantic species Arild JohnsenEirik RindalPer G. P. EricsonDario Zuccon • Kevin C. R. KerrMark Y. StoeckleJan T. Lifjeld
TL;DR: In this paper, the authors performed a compre- hensive COI (cytochrome c oxidase subunit I) barcode survey of 296 species of Scandinavian birds and compared genetic divergences among 78 trans-Atlantic species whose breeding ranges include both Scandinavia and North America.
Journal ArticleDOI
An overview to the investigative approach to species testing in wildlife forensic science
Adrian Linacre,Shanan S. Tobe +1 more
TL;DR: The aim of this review is to illustrate aspects of species testing in wildlife forensic science and to explain how a knowledge of genetic variation at the genus and species level can aid in the reporting of results.
Journal ArticleDOI
Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans
Jorge Lobo,Jorge Lobo,Pedro M. Costa,Marcos A. L. Teixeira,Maria S. G. Ferreira,Maria Helena Costa,Filipe O. Costa +6 more
TL;DR: These primers demonstrated to be highly cost-effective, which is of key importance for DNA barcoding procedures, such as for building comprehensive DNA barcode libraries of marine communities, where the processing of a large numbers of specimens from a wide variety of marine taxa is compulsory.
Journal ArticleDOI
Cryptic genetic diversity in "widespread" Southeast Asian bird species suggests that Philippine avian endemism is gravely underestimated.
David J. Lohman,David J. Lohman,Krista K. Ingram,Dewi M. Prawiradilaga,Kevin Winker,Frederick H. Sheldon,Robert G. Moyle,Peter K. L. Ng,Perry S. Ong,Luan Keng Wang,Thomas M. Braile,Thomas M. Braile,Dwi Astuti,Rudolf Meier +13 more
TL;DR: Genetic and phylogenetic evidence is presented corroborated by morphology that Philippine populations of seven widespread, non-migratory passerine birds might represent unrecognized, distinct species, and an extrapolation suggests that the proportion of endemic bird species in the Philippines could be much higher than currently estimated.
References
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Journal Article
DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.
TL;DR: "universal"
Journal ArticleDOI
Biological identifications through DNA barcodes
TL;DR: It is established that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals and will provide a reliable, cost–effective and accessible solution to the current problem of species identification.
Journal ArticleDOI
Cryptic species as a window on diversity and conservation
David Bickford,David J. Lohman,Navjot S. Sodhi,Peter K. L. Ng,Rudolf Meier,Kevin Winker,Krista K. Ingram,Indraneil Das +7 more
TL;DR: The literature on cryptic and sibling species is synthesized and trends in their discovery are discussed, suggesting that the discovery of cryptic species is likely to be non-random with regard to taxon and biome and could have profound implications for evolutionary theory, biogeography and conservation planning.
Journal ArticleDOI
Identification of Birds through DNA Barcodes
TL;DR: The finding of large COI sequence differences between, as compared to small differences within, species confirms the effectiveness of COI barcodes for the identification of bird species, and implies that a standard screening threshold of sequence difference could speed the discovery of new animal species.
Journal ArticleDOI
DNA barcodes distinguish species of tropical Lepidoptera
TL;DR: It is shown that cytochrome c oxidase I DNA barcodes effectively discriminate among species in three Lepidoptera families from Area de Conservación Guanacaste in northwestern Costa Rica, suggesting DNA barcoding will significantly aid species identification and discovery in tropical settings.