Open AccessJournal Article
DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.
TLDR
"universal"Abstract:
We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, Pogonophora, Arthropoda, Nemertinea, Echiura, Sipuncula, Platyhelminthes, Tardigrada, and Coelenterata, as well as the putative phylum Vestimentifera. Preliminary comparisons revealed that these COI primers generate informative sequences for phylogenetic analyses at the species and higher taxonomic levels.read more
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Biological identifications through DNA barcodes
TL;DR: It is established that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals and will provide a reliable, cost–effective and accessible solution to the current problem of species identification.
Journal ArticleDOI
Who is eating what: diet assessment using next generation sequencing.
François Pompanon,Bruce E. Deagle,Bruce E. Deagle,William Oliver Christian Symondson,David Steven Brown,Simon N. Jarman,Pierre Taberlet +6 more
TL;DR: The power and pitfalls of NGS diet methods are reviewed, the critical factors to take into account when choosing or designing a suitable barcode are presented and the validation of data accuracy including the viability of producing quantitative data is discussed.
Journal ArticleDOI
A new versatile primer set targeting a short fragment of the mitochondrial COI region for metabarcoding metazoan diversity: application for characterizing coral reef fish gut contents
Matthieu Leray,Matthieu Leray,Joy Y. Yang,Christopher P. Meyer,Suzanne C. Mills,Natalia Agudelo,Vincent Ranwez,J. T. Boehm,J. T. Boehm,Ryuji J. Machida +9 more
TL;DR: The molecular analysis of gut contents targeting the 313 COI fragment using the newly designed mlCOIintF primer in combination with the jgHCO2198 primer offers enormous promise for metazoan metabarcoding studies.
Journal ArticleDOI
Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplified
TL;DR: It is demonstrated that the presence of COI numts makes this goal difficult to achieve when numts are prevalent and can introduce serious ambiguity into DNA barcoding, which strives for rapid and inexpensive generation of molecular species tags.
References
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Journal ArticleDOI
Sequence and organization of the human mitochondrial genome
Stephen Anderson,Alan T. Bankier,Bart Barrell,M.H.L. de Bruijn,Alan Coulson,J. Drouin,J. Drouin,Ian C. Eperon,Donald P. Nierlich,Donald P. Nierlich,Bruce A. Roe,Bruce A. Roe,Frederick Sanger,P. H. Schreier,Andrew J.H. Smith,Rodger Staden,Ian G. Young,Ian G. Young +17 more
TL;DR: The complete sequence of the 16,569-base pair human mitochondrial genome is presented and shows extreme economy in that the genes have none or only a few noncoding bases between them, and in many cases the termination codons are not coded in the DNA but are created post-transcriptionally by polyadenylation of the mRNAs.
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Sequence and gene organization of mouse mitochondrial DNA
TL;DR: The mouse mitochondrial DNA genome is highly homologous in overall sequence and in gene organization to human mitochondrial DNA, with the descending order of conserved regions being tRNA genes; origin of light-strand replication; r RNA genes; knownprotein-coding genes; unidentified protein-c coding genes; displacement-loop region.
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The mitochondrial DNA molecular of Drosophila yakuba: nucleotide sequence, gene organization, and genetic code.
TL;DR: Evidence is summarized that supports the hypothesis that A and T nucleotides are favored at all locations in the D. yakuba mtDNA molecule where these nucleotide are compatible with function.
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Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome.
TL;DR: The bovine 12 S and 16 S Ribosomal RNA genes, when compared with those from human mitochondrial DNA, show conserved features that are consistent with proposed secondary structure models for the ribosomal RNAs.
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Sequence and gene organization of the chicken mitochondrial genome. A novel gene order in higher vertebrates.
Paul Desjardins,Réjean Morais +1 more
TL;DR: The 16,775 base-pair mitochondrial genome of the white leghorn chicken has been cloned and sequenced as discussed by the authors, which exhibits a novel gene order, the contiguous tRNAGlu and ND6 genes are located immediately adjacent to the displacement loop region of the molecule, just ahead of the contiguous TRNAPro, tRNAThr and cytochrome b genes.