Journal ArticleDOI
Improvement in Semen Quality Using Glass Bead Column
Salim Daya,R. B. L. Awatkin +1 more
TLDR
A simple procedure that uses a column of glass beads to separate spermatozoa from poor quality semen is described, which has significantly better progressive motility and viability and also have enhanced fertilizing capacity.Abstract:
Fertilizing ability of semen requires the presence of viable and motile spermatozoa whose surface-membrane integrity is maintained. Male infertility is often the result of these variables being suboptimal. Various in vitro methods have been reported that separate good-quality spermatozoa from such ejaculates. In this study we describe a simple procedure that uses a column of glass beads to separate spermatozoa from poor quality semen. The filtered spermatozoa have significantly better progressive motility and viability and also have enhanced fertilizing capacity. Such a method may have clinical use for semen preparation prior to intrauterine insemination and in vitro fertilization. It may also have a role in the assessment of semen to determine fertilization potential and hence provide prognostic information to infertile couples.read more
Citations
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Journal ArticleDOI
Effect of zinc on human sperm motility and the acrosome reaction
M. Riffo,S. Leiva,J. Astudillo +2 more
TL;DR: Results suggest that extracellular zinc acts as an inhibitor of human sperm motility and the acrosome reaction (and/or capacitation and the acupuncture reaction) which is reversible and occurs in a dose-dependent fashion.
Journal ArticleDOI
Sephadex and sephadex ion-exchange filtration improves the quality and freezability of low-grade buffalo semen ejaculates
TL;DR: It is concluded that filtration systems containing an FS+IE column can effectively enhance the quality and freezability of extended, low quality buffalo semen.
Journal ArticleDOI
Role of phospholipase A2 in mammalian sperm-egg fusion: Development of hamster oolemma fusibility by lysophosphatidylcholine
Marta S. Riffo,Mario Párraga +1 more
TL;DR: It is suggested that sperm PLA2 and one of its modulators, the LPC, may contribute to membrane-fusion events in mammalian fertilization.
Journal ArticleDOI
Study of the acrosome reaction and the fertilizing ability of hamster epididymal cauda spermatozoa treated with antibodies against phospholipase A2 and/or lysophosphatidylcholine.
Marta S. Riffo,Mario Párraga +1 more
TL;DR: Experiments in vitro showed that LPC is able to reverse the inhibitory effects of the antibodies on the acrosome reaction and fertilization, suggesting that PLA2 and one of its reaction products might contribute to membrane-fusion events during mammalian fertilization.
Journal ArticleDOI
Filtration of bovine semen. I. Development of a Sephadex ion-exchange filter
M. Anzar,E.F. Graham +1 more
TL;DR: In this article, a Sephadex ion-exchange filter was used to improve the quality of bovine semen by adding a fixed quantity of Sephadesadex (0.6 g), with a varied quantity of ionexchanger, i.e. diethylaminoethane (DEAE) and carboxy-methyl (CM) celluloses (0, 0.25 or 0.50 g).
References
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Journal ArticleDOI
Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics
TL;DR: The hypoosmotic swelling technique to evaluate the functional integrity of the sperm membrane appears to give high repeatability and accuracy and is closely correlated to the in-vitro fertilizing ability of spermatozoa.
Journal ArticleDOI
Intrauterine insemination: a critical review.
Edward E. Wallach,Nancy C. Allen,Carl M. Herbert,Wayne S. Maxson,B. Jane Rogers,Michael P. Diamond,Anne Colston Wentz +6 more
Journal ArticleDOI
Improved conception rate after intrauterine insemination of washed spermatozoa from men with poor quality semen.
TL;DR: The efficacy of high intrauterine insemination of a washed motile fraction of spermatozoa from men with poor quality semen on the day after the luteinising hormone (LH) surge was compared with that of natural intercourse based on symptothermal methods and a single act of intercourse timed in the same 35 couples in a controlled and randomised trial of the three types of cycle.
Journal ArticleDOI
Correlation between human sperm swelling in hypoosmotic medium (hypoosmotic swelling test) and in vitro fertilization.
H. van der Ven,R. S. Jeyendran,Safaa Al-Hasani,M. Perez-Pelaez,Klaus Diedrich,Lourens J.D. Zaneveld +5 more
TL;DR: It appears that the hypoosmotic swelling test is a more accurate predictor of successful in vitro fertilization outcome than the conventional semen parameters.
Journal ArticleDOI
Effects of human seminal plasma on fertilizing capacity of human spermatozoa.
Kuldip C. Kanwar,Kuldip C. Kanwar,Ryuzo Yanagimachi,Ryuzo Yanagimachi,Alexander Lopata,Alexander Lopata +5 more
TL;DR: In this paper, the effects of human seminal plasma on the fertilizing capacity of human spermatozoa were investigated using zona-free hamster eggs and salt-stored human eggs.
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