Journal ArticleDOI
In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.
TLDR
The following two articles describe successful syntheses in vitro of DNA copies of various mammalian globin mRNAs, using reverse transcriptase from avian myoblastosis virus, using poly(dT) sequences at their 3′-ends.Abstract:
The following two articles describe successful syntheses in vitro of DNA copies of various mammalian globin mRNAs, using reverse transcriptase from avian myoblastosis virus. Short stretches of poly(dT) greatly enhance this effect, presumably because they prime correct initiation of reverse transcription of these mRNAs by pairing with known poly (A) sequences at their 3′-ends.read more
Citations
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Journal ArticleDOI
Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.
G S McKnight,Richard D. Palmiter +1 more
TL;DR: Relative rates of ovalbumin and conalbumin mRNA transcription were measured in isolated oviduct nuclei by allowing endogenous RNA polymerases to synthesize [32P]RNA that was then hybridized to immobilized recombinant DNA containing the respective gene sequences.
Journal ArticleDOI
Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.
TL;DR: The utility of this nuclease for measuring annealing of labeled DNA is discussed and the enzyme is shown to be absolutely specific for single-stranded nucleic acids.
Journal ArticleDOI
Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus.
A Chien,D B Edgar,J M Trela +2 more
TL;DR: A stable deoxyribonucleic acid (DNA) polymerase with a temperature optimum of 80 degrees C has been purified from the extreme thermophile Thermus aquaticus and is distinguished from the DNA polymerase of Escherichia coli.
Journal ArticleDOI
Three abundance classes in HeLa cell messenger RNA
TL;DR: Approximately 35,000 different poly(A)-containing RNA sequences are present in HeLa cell cytoplasm and the sequences are grouped in three distinct abundance classes.
Book
Advances in Human Genetics
Harry Harris,Kurt Hirschhorn +1 more
TL;DR: This book has five chapters covering peroxisomal diseases, X-linked immunodeficiencies, genetic mutations affecting human lipoproteins and their receptors and enzymes, genetic aspects of cancer, and Gaucher disease.
References
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Journal ArticleDOI
Viral RNA-dependent DNA Polymerase: RNA-dependent DNA Polymerase in Virions of RNA Tumour Viruses
TL;DR: Two independent groups of investigators have found evidence of an enzyme in virions of RNA tumour viruses which synthesizes DNA from an RNA template, apparently the classical process of information transfer from DNA to RNA can be inverted.
Journal ArticleDOI
RNA-dependent DNA polymerase in virions of Rous sarcoma virus.
Howard M. Temin,Satoshi Mizutani +1 more
TL;DR: Viral RNA-dependent DNA Polymerase: RNA- dependent DNA polymerase in Virions of Rous Sarcoma Virus and its role in cell reprograming is studied.
Journal ArticleDOI
Characterization of macromolecules by constant velocity sedimentation.
TL;DR: To exploit the analytical power of zone velocity centrifugation, a novel method has been developed using sucrose gradients calculated to give constant particle velocity.
Journal ArticleDOI
Ribonucleic Acid Synthesis of Vesicular Stomatitis Virus, II. An RNA Polymerase in the Virion
TL;DR: The virions of vesicular stomatitis virus contain an enzyme that catalyzes the incorporation of ribonucleotides into RNA, and the product of the reaction is mainly RNA complementary in base sequence to that of veS virus RNA.
Journal ArticleDOI
Adenine-rich polymer associated with rabbit reticulocyte messenger RNA.
L Lim,E. S. Canellakis +1 more
TL;DR: The isolation of an adenine-rich polymer from purified messenger RNA obtained from rabbit reticulocyte polysomes is described and its relationship to the size of the ribosomes from which the messenger RNA is derived is described.
Related Papers (5)
Viral RNA-dependent DNA Polymerase: RNA-dependent DNA Polymerase in Virions of RNA Tumour Viruses
RNA-dependent DNA polymerase in virions of Rous sarcoma virus.
Howard M. Temin,Satoshi Mizutani +1 more
Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
Haim Aviv,Philip Leder +1 more