Inactivation of glucagon by plasma membranes of rat liver.
TLDR
Liver plasma membranes may be an important site for specific inactivation of glucagon, and a possible relationship between the glucagon inactivation process and the receptor for glucagon is discussed.About:
This article is published in Journal of Biological Chemistry.The article was published on 1972-04-25 and is currently open access. It has received 102 citations till now. The article focuses on the topics: Glucagon binding & Glucagon.read more
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Book ChapterDOI
Membrane receptors and hormone action.
TL;DR: This chapter describes the relation between biological activity and binding, physicochemical detection and assay of receptors, solubilization of membrane receptors, examples of membrane-localized receptors, affinity techniques, polyvalent hormone derivatives, and receptor studies, and theoretical mechanisms of hormone–receptor binding and action.
Journal ArticleDOI
Peptide hormone binding to receptors: a review of direct studies in vitro.
TL;DR: It will be clear that scientists now working with peptide hormone receptors have appropriated directly methods and technical approaches that were devised by Berson and Yalow for radioimmunoassay for the development ofThis paper describes how the approach has been extended by dozens of laboratories to many other peptide hormones.
Journal ArticleDOI
Properties of angiotensin II receptors in the bovine and rat adrenal cortex.
TL;DR: The presence of high affinity specific binding sites for angiotensin II in bovine and rat adrenal cortex and suggest a plasma membrane location for the angiotENSin II receptors are illustrated.
Journal ArticleDOI
Binding of 125I-human growth hormone to specific receptors in human cultured lymphocytes. Characterization of the interaction and a sensitive radioreceptor assay.
TL;DR: Binding of 125I-human growth hormone to cultured lymphocytes was relatively insensitive to alterations in the pH and in the concentrations of Ca2+, Mg2+, or EDTA, and the potencies of the human growth hormone preparations determined by the radioreceptor assay correlated more closely with the bioassay than with the radioimmunoassay.
Journal ArticleDOI
Interaction of porcine vasoactive intestinal peptide with dispersed pancreatic acinar cells from the guinea pig. Binding of radioiodinated peptide.
TL;DR: The dose-response curve for inhibition of 125I-VIP binding by VIP or secretin was biphasic and suggested that pancreatic acinar cells have two classes of binding sites: (a) a relatively small number of sites with a high affinity for VIP and a low affinity for secretin, and (b) a comparatively large number of Sites with a low affinities for VIP
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
W M Hunter,F. C. Greenwood +1 more
TL;DR: Current procedures for the immunological assay of protein hormones in human plasma require the routine preparation of hormones labelled with iodine-131 of high specific activity, and this work demonstrates the importance of knowing the carrier and removal status of iodine.
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Studies of pancreatic alpha cell function in normal and diabetic subjects
TL;DR: The findings suggest that alpha cell function is inappropriately increased in diabetes mellitus and could play a significant role in the diabetic syndrome.
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Isolation of an organ specific protein antigen from cell-surface membrane rat liver
TL;DR: The liver membrane protein could not be detected in extracts of Morris hepatoma but was found to be present in a highly differentiated second generation hepatoma induced by diacetyl amino fluorene.
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The Glucagon-sensitive Adenyl Cyclase System in Plasma Membranes of Rat Liver V. AN OBLIGATORY ROLE OF GUANYL NUCLEOTIDES IN GLUCAGON ACTION
TL;DR: It is concluded that guanyl nucleotides play a specific and obligatory role in the activation of adenyl cyclase by glucagon, and bind at sites, distinct from the glucagon binding sites, that appear to regulate both the response ofadenyl cycling to glucagon and the actions of fluoride ion on this system.