Journal ArticleDOI
Increased production of withanolide A, withanone, and withaferin A in hairy root cultures of Withania somnifera (L.) Dunal elicited with methyl jasmonate and salicylic acid
Ganeshan Sivanandhan,Gnanajothi Kapil Dev,Murugaraj Jeyaraj,Manoharan Rajesh,Annavi Arjunan,Manickam Muthuselvam,Markandan Manickavasagam,Natesan Selvaraj,Andy Ganapathi +8 more
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TLDR
A higher production of withanolide A, withanone and withaferin A from the elicited-hairy roots of W. somnifera under optimal inoculum mass, harvest time, elicitor exposure time and its concentration is reported.Abstract:
Withania somnifera, an important medicinal plant that possesses a variety of bioactive secondary metabolites collectively known as withanolides. Hairy roots with an initial inoculum mass of 5 g FW were elicited separately with methyl jasmonate (MeJ) and salicylic acid (SA) at various concentrations for different exposure times after 30 days of culture. Enhanced production of biomass (32.68 g FW and 5.54 g DW; 1.23-fold higher), withanolide A (132.44 mg/g DW; 58-fold higher), withanone (84.35 mg/g DW; 46-fold higher), and withaferin A (70.72 mg/g DW; 42-fold higher) were achieved from 40 day-old harvested hairy roots elicited with 150 μM SA for 4 h exposure time. The present study reports a higher production of withanolide A, withanone and withaferin A from the elicited-hairy roots of W. somnifera under optimal inoculum mass, harvest time, elicitor exposure time and its concentration. These results will be useful for biochemical and bioprocess engineering for the viable production of withanolides in hairy root culture.read more
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Journal ArticleDOI
Improving production of plant secondary metabolites through biotic and abiotic elicitation
TL;DR: Different elicitation methods through various biotic (proteins, carbohydrates, plant growth promoting rhizobacteria, fungus, hormones) and abiotic elicitors that could bring about increased yield of secondary metabolites especially in medicinal plants beneficial to the medicinal and aromatic industry are discussed.
Journal ArticleDOI
Elicitation: a stimulation of stress in in vitro plant cell/tissue cultures for enhancement of secondary metabolite production
M. Narayani,Smita Srivastava +1 more
TL;DR: Integration of transcriptomics, proteomics and metabolomics with system biology can aid in discovery of novel genes, transcriptional factors and several biosynthetic pathways which in turn can serve as a valuable tool for metabolic engineering and gene manipulation for enhancing the yield and productivity of secondary metabolites.
Journal ArticleDOI
Plant Secondary Metabolite Biosynthesis and Transcriptional Regulation in Response to Biotic and Abiotic Stress Conditions
TL;DR: The shikimate pathway and the aromatic amino acids produced in this pathway are the precursors of a range of secondary metabolites including terpenoids, alkaloids, and sulfur- and nitrogen-containing compounds and how the biosynthesis of important metabolites is altered by several genes related to secondary metabolite biosynthesis pathways are detailed.
Book ChapterDOI
Abiotic and Biotic Elicitors–Role in Secondary Metabolites Production through In Vitro Culture of Medicinal Plants
TL;DR: This chapter reviews the various abiotic and biotic elicitors applied to cultural system and their stimulating effects on the accumulation of secondary metabolites.
Journal ArticleDOI
Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures
TL;DR: This is a comprehensive review about the progress in the elicitation approach to hairy root cultures from 2010 to 2019 and the information provided is valuable and will be of interest for scientists working in this area of plant biotechnology.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
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TL;DR: This review summarizes biosynthesis and signal transduction of jasmonates with emphasis on new findings in relation to enzymes, their crystal structure, new compounds detected in the oxylipin andJasmonate families, and newly found functions.
Journal ArticleDOI
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TL;DR: Progress made on several aspects of elicitor signal transduction leading to production of plant secondary metabolites are summarized, including the integration of multiple signaling pathways into or by transcription factors, as well as the linkage of the above signal components in eliciting network through protein phosphorylation and dephosphorylation.
Journal ArticleDOI
Plant cell cultures: Chemical factories of secondary metabolites
TL;DR: The production of the pungent food additive capsaicin, the natural colour anthocyanin and the natural flavour vanillin is described in detail.
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