Open AccessJournal Article
Induction of phytoalexins biosynthesis in orange fruit by the biocontrol yeast Rhodotorula glutinis.
Arras G,Guy D'hallewin,Maria Giovanna Molinu,Antonio Dore,Venditti T,M. Fois,G Lima,Mario Carlo Salvatore Agabbio +7 more
TLDR
The biocontrol yeast Rhodotorula glutinis, isolate 21A, obtained from tomato fruit was used to control Penicillium digitatum, P. italicum and Botrytis cinerea on artificially wounded citrus fruit, revealing a fast colonization of the growing fungal mycelia by the yeast cells, but no sign of lytic activity on hyphae was observed.Abstract:
The biocontrol yeast Rhodotorula glutinis, isolate 21A, obtained from tomato fruit was used to control Penicillium digitatum, P. italicum and Botrytis cinerea on artificially wounded citrus fruit. Orange and satsuma mandarin fruit were treated with the biocontrol yeast, inoculated with the pathogens and stored for 7 days at 23 degrees C. On orange fruit the antagonist compared to the control reduced decay by 92.2, 88.4 and 96.2% for P. digitatum, P. italicum and B. cinerea, respectively. On satsuma mandarin fruit the same pathogens were inhibited by 96.2, 91.2 and 90.0%, respectively. Scanning electron microscope observations, focusing on the antagonist-pathogen interactions, revealed a fast colonization of the growing fungal mycelia by the yeast cells, but no sign of lytic activity on hyphae was observed. Moreover, the fruit accumulated the phytoalexins scoparone and scopoletin into artificial wounds previously treated by the yeast and either inoculated or uninoculated with the pathogen. The concentration of scoparone, which showed higher accumulation in fruit tissues, varied significantly in relation to the time lag between the application of the antagonist and the inoculation with the pathogen. In particular, the concentration of scoparone 4 days after application of the sole yeast was 69.0 microg x g(-1) fresh weight (FW), 6.3 times higher than in the uninoculated wounded tissues (11.0 microg x g(-1) FW). The phytoalexin accumulation was low (13.0 microg x g(-1)FW) applying the yeast jointly with P. digitatum into wounds, while it increased consistently (74.0 microg x g(-1)FW) when the antagonist was applied 24 h before the pathogen.read more
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Coumarins from the peel of citrus grown in Colombia: composition, elicitation and antifungal activity.
TL;DR: Analysis of peels from fruits of different citrus cultivated in Colombia shows that isolated coumarins could be involved in the defense mechanisms of C. latifolia, C. aurantifolia and C. limonia and their accumulation may be modulated by the application of elicitors.
Journal ArticleDOI
Effect of scoparone on neurite outgrowth in PC12 cells.
TL;DR: It is suggested that scoparone can induce neurite outgrowth by stimulating the upstream steps of ERK, PKA, PKC and CaMK II in PC12 cells.
Journal ArticleDOI
Effects of scoparone on dopamine biosynthesis and L‐DOPA‐induced cytotoxicity in PC12 cells
Yoo Jung Yang,Hak Ju Lee,Hai Shan Huang,Byung Koo Lee,Hyun Sook Choi,Sung Cil Lim,Chong-Kil Lee,Myung Koo Lee +7 more
TL;DR: Results suggest that scoparone enhances dopamine biosynthesis by regulating TH activity and TH gene expression, which is mediated by the PKA, CREB, PKC, and CaMK II pathways, and protects cells from L‐DOPA‐induced cytotoxicity by inducing cyclic AMP‐PKA systems in PC12 cells.
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Antifungal activity against Colletotrichum acutatum and Colletotrichum gloeosporioides of the major constituents from wood sawdust of Platymiscium gracile Benth
TL;DR: In the present work, the in vitro antifungal activity of the soluble fractions in n- hexane, dichloromethane, and ethyl acetate, and their major constituents from the sawdust of timber specie Platymiscium gracile Benth.
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Accumulation of Defence Response‐related and Unique Expressed Sequence Tags during the Incompatible Interaction in the Oryza sativa–Magnaporthe oryzae Pathosystem
Rekha Dixit,Ashutosh Bhargava,Vivek Dalal,Prikshit Plaha,Nagendera Kumar Singh,Tilak Raj Sharma +5 more
TL;DR: The unigenes obtained in this study were physically located on the pseudomolecules of rice genome and can be used for determining the arrays of genes being expressed during Oryza sativa―M.
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