Inhibitors of sterol synthesis. Submicromolar 14 alpha-ethyl-5 alpha-cholest-7-ene-3 beta, 15 alpha-diol causes a major modification of the sterol composition of CHO-K1 cells and a marked change in cell morphology.
A Izumi,Frederick D. Pinkerton,S. O. Nelson,J. S. Pyrek,P. J. G. Neill,J. H. Smith,G. J. Schroepfer +6 more
TLDR
It is demonstrated that marked changes in the sterol composition of CHO-K1 cells can be effected by incubation of the cells with 14 alpha-ethyl-5 alpha-cholest-7-ene-3 beta, 15 alpha-diol, miconazole, or lanosterol, and an important role of sterols in the control of the shape of these cells is indicated.About:
This article is published in Journal of Lipid Research.The article was published on 1994-07-01 and is currently open access. It has received 7 citations till now. The article focuses on the topics: Sterol & Lanosterol.read more
Citations
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Journal ArticleDOI
Oxysterols: modulators of cholesterol metabolism and other processes.
TL;DR: This review comprises a detailed and critical assessment of current knowledge regarding the formation, occurrence, metabolism, regulatory properties, and other activities of oxysterols in mammalian systems.
Journal ArticleDOI
Review of progress in sterol oxidations: 1987–1995
TL;DR: Material dealing with the chemistry, biochemistry, and biological activities of oxysterols is reviewed for the period 1987-1995 and particular attention is paid to the presence of oxystersols in tissues and foods and to their physiological relevance.
Journal ArticleDOI
Analysis of unsaturated C27 sterols by nuclear magnetic resonance spectroscopy.
William K. Wilson,Rhea Sumpter,Joshua J. Warren,Peter S. Rogers,Benfang Ruan,George J. Schroepfer +5 more
TL;DR: Complete 1H and 13C nuclear magnetic resonance (NMR) signal assignments have been established for 5 alpha-cholestan-3 beta-ol, 22 unsaturated C27 sterols, and their acetate derivatives and indicate considerable conformational diversity, which was confirmed by molecular modeling.
Journal ArticleDOI
Sterol synthesis. A timely look at the capabilities of conventional and silver ion high performance liquid chromatography for the separation of C27 sterols related to cholesterol biosynthesis
TL;DR: These studies indicate very limited capabilities of reversed phase and normal phase HPLC for the separation of C27 sterols differing in the number and location of olefinic double bonds, while silver ion HPLC provided remarkable separations of the same compounds, either as the free sterols or their acetate derivatives.
Journal ArticleDOI
Silver ion high pressure liquid chromatography provides unprecedented separation of sterols: application to the enzymatic formation of cholesta-5,8-dien-3 beta-ol.
TL;DR: It is reported that silver ion HPLC provides remarkable separations of C27 sterols differing only in the number or location of olefinic double bonds.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY
TL;DR: The stain reported here differs from previous alkaline lead stains in that the chelating agent, citrate, is in sufficient excess to sequester all lead present, and is less likely to contaminate sections.
Journal ArticleDOI
A low-viscosity epoxy resin embedding medium for electron microscopy.
TL;DR: A low-viscosity embedding medium based on ERL-4206 is recommended for use in electron microscopy and has a long pot life of several days and infiltrates readily because of its low viscosity.
Journal ArticleDOI
A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity.
TL;DR: The simplified but precise method described in this paper involves treatment of the serum with alcoholic potassium hydroxide to liberate the cholesterol from the lipoprotein complexes and to saponify the cholesterol esters.
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