Intrinsic optical and passive electrical properties of cut frog twitch fibers.
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TLDR
The intrinsic fiber absorbance, defined operationally as log10 of the ratio of incident light to transmitted light intensity, was similar in intact and cut preparations, as were the changes that accompanied stimulation; changes are probably due to a progressive alteration in the internal state of the cut fibers.Abstract:
�This article describes a new apparatus for making simultaneous optical measurements on single muscle fibers at three different wavelengths and two planes of linear polarization There are two modes of operation : mode 1 measures the individual absorbances of light linearly polarized along and perpendicular to the fiber axis, and mode 2 measures retardation (or birefringence) and the average of the two absorbance components Although some intact frog twitch fibers were studied, most experiments used cut fibers (Hille, B , and D T Campbell 1976 Journa l of General Physiology 67:265-293) mounted in a double-Vaseline-gap chamber (Kovacs, L , E Rios, and M F read more
Citations
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Voltage sensor of excitation-contraction coupling in skeletal muscle.
Eduardo Ríos,Gonzalo Pizarro +1 more
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Two mechanisms of quantized calcium release in skeletal muscle
Michael G. Klein,Heping Cheng,Luis Fernando Santana,Y. H. Jiang,W. J. Lederer,Martin F. Schneider +5 more
TL;DR: A 'dual-control' model for discrete Ca2+ release events from the sacroplasmic reticulum is proposed that unifies diverse observations about Ca2-signalling in frog skeletal muscle, and that may be applicable to other excitable cells.
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Model of Sarcomeric Ca2+ Movements, Including ATP Ca2+ Binding and Diffusion, during Activation of Frog Skeletal Muscle
TL;DR: It is concluded that Ca2+ binding by ATP and diffusion of CaATP make important contributions to the determination of the amplitude and the time course of Δ[Ca2+].
Journal ArticleDOI
Use of fura red as an intracellular calcium indicator in frog skeletal muscle fibers
TL;DR: Fura red, a fluorescent Ca2+ indicator with absorbance bands at visible wavelengths, was injected into intact single muscle fibers that had been stretched to a long sarcomere length and bathed in a 'high-Ca2+' Ringer and it was estimated that approximately 85% of the indicator molecules are bound to muscle constituents of large molecular weight.
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Ca2+ release from the sarcoplasmic reticulum compared in amphibian and mammalian skeletal muscle
TL;DR: Signs of fundamental differences in control mechanisms between mammalian and amphibian muscle are derived from the marked differences in voltage dependence of the ratio, as well as consideration of Ca(2+)-induced release models.
References
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Book ChapterDOI
Fitting and Statistical Analysis of Single-Channel Records
David Colquhoun,Fred J. Sigworth +1 more
TL;DR: The aims of analysis of single channel records can be considered in two categories: to allow one to observe results at leisure in order to determine their qualitative features and to allow highly automated methods of analysis to be fulfilled.
Journal ArticleDOI
An improved vaseline gap voltage clamp for skeletal muscle fibers.
Bertil Hille,Donald T. Campbell +1 more
TL;DR: Comparison of recorded currents with simulations shows that while the transverse tubular system has regenerative sodium currents, they are too small to make important errors in the total current recorded at the surface under voltage clamp at low temperature.
Journal ArticleDOI
Measurement and modification of free calcium transients in frog skeletal muscle fibres by a metallochromic indicator dye.
TL;DR: Myoplasmic free calcium transients were monitored with the metallochromic indicator dye Antipyrylazo III in single frog skeletal muscle fibres cut at both ends, stretched so as to minimize or eliminate contractile filament overlap and voltage clamped using a double‐Vaseline‐gap system.
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An improved vaseline gap voltage clamp for skeletal muscle fibers.
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