Involvement of a Novel Efflux System in Biofilm-Specific Resistance to Antibiotics
Li Zhang,Thien-Fah Mah +1 more
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A novel efflux pump in Pseudomonas aeruginosa is identified that is important for biofilm-specific resistance to a subset of antibiotics and combining the ndvB mutation with the PA1874-1877 gene deletion results in a mutant strain that is more sensitive to antibiotics than either single mutant strain.Abstract:
Bacteria growing in biofilms are more resistant to antibiotics than their planktonic counterparts. How this transition occurs is unclear, but it is likely there are multiple mechanisms of resistance that act together in order to provide an increased overall level of resistance to the biofilm. We have identified a novel efflux pump in Pseudomonas aeruginosa that is important for biofilm-specific resistance to a subset of antibiotics. Complete deletion of the genes encoding this pump, PA1874 to PA1877 (PA1874-1877) genes, in an P. aeruginosa PA14 background results in an increase in sensitivity to tobramycin, gentamicin, and ciprofloxacin, specifically when this mutant strain is growing in a biofilm. This efflux pump is more highly expressed in biofilm cells than in planktonic cells, providing an explanation for why these genes are important for biofilm but not planktonic resistance to antibiotics. Furthermore, expression of these genes in planktonic cells increases their resistance to antibiotics. We have previously shown that ndvB is important for biofilm-specific resistance (T. F. Mah, B. Pitts, B. Pellock, G. C. Walker, P. S. Stewart, and G. A. O'Toole, Nature 426:306-310, 2003). Our discovery that combining the ndvB mutation with the PA1874-1877 gene deletion results in a mutant strain that is more sensitive to antibiotics than either single mutant strain suggests that ndvB and PA1874-1877 contribute to two different mechanisms of biofilm-specific resistance to antibiotics.read more
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References
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Thien-Fah Mah,George A. O'Toole +1 more
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Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development
George A. O'Toole,Roberto Kolter +1 more
TL;DR: The isolation and characterization of mutants of Pseudomonas aeruginosa PA14 defective in the initiation of biofilm formation on an abiotic surface, polyvinylchloride (PVC) plastic are reported and evidence that microcolonies form by aggregation of cells present in the monolayer is presented.
Journal ArticleDOI
Pseudomonas aeruginosa Displays Multiple Phenotypes during Development as a Biofilm
TL;DR: The results demonstrate that P. aeruginosa displays multiple phenotypes during biofilm development and that knowledge of stage-specific physiology may be important in detecting and controlling biofilm growth.
Book
Short protocols in molecular biology : a compendium of methods from Current protocols in molecular biology
TL;DR: Discovery and Analysis of Differentially Expressed Genes in Single Cells and Cell Populations Appendices and Informatics for Molecular Biologists.