Isolation and Complete Genome Sequence of Algibacter alginolytica sp. nov., a Novel Seaweed-Degrading Bacteroidetes Bacterium with Diverse Putative Polysaccharide Utilization Loci
Cong Sun,Geyi Fu,Chongya Zhang,Jing Hu,Lin Xu,Ruijun Wang,Yue Su,Shuai-Bo Han,Xiao-Yun Yu,Hong Cheng,Xin-Qi Zhang,Ying-Yi Huo,Xue-Wei Xu,Min Wu +13 more
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TLDR
This work reveals that strain HZ22T has good potential for the degradation of algal polysaccharides and that the structure and related mechanism of PUL in strain Hz22T are worth further research.Abstract:
The members of the phylum Bacteroidetes are recognized as some of the most important specialists for the degradation of polysaccharides. However, in contrast to research on Bacteroidetes in the human gut, research on polysaccharide degradation by marine Bacteroidetes is still rare. The genus Algibacter belongs to the Flavobacteriaceae family of the Bacteroidetes, and most species in this genus are isolated from or near the habitat of algae, indicating a preference for the complex polysaccharides of algae. In this work, a novel brown-seaweed-degrading strain designated HZ22 was isolated from the surface of a brown seaweed (Laminaria japonica). On the basis of its physiological, chemotaxonomic, and genotypic characteristics, it is proposed that strain HZ22 represents a novel species in the genus Algibacter with the proposed name Algibacter alginolytica sp. nov. The genome of strain HZ22, the type strain of this species, harbors 3,371 coding sequences (CDSs) and 255 carbohydrate-active enzymes (CAZymes), including 104 glycoside hydrolases (GHs) and 18 polysaccharide lyases (PLs); this appears to be the highest proportion of CAZymes (∼7.5%) among the reported strains in the class Flavobacteria Seventeen polysaccharide utilization loci (PUL) are predicted to be specific for marine polysaccharides, especially algal polysaccharides from red, green, and brown seaweeds. In particular, PUL N is predicted to be specific for alginate. Taking these findings together with the results of assays of crude alginate lyases, we prove that strain HZ22(T) can completely degrade alginate. This work reveals that strain HZ22(T) has good potential for the degradation of algal polysaccharides and that the structure and related mechanism of PUL in strain HZ22(T) are worth further research.read more
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Marine Polysaccharide Sulfatases
TL;DR: The modularity of sulfatases and their location in marine polysaccharide utilization loci (PUL) provide clues as to their potential substrates and can drive future functional assays, and the low number of currently biochemically characterized marine carbohydrate sulf atases is highlighted.
Journal ArticleDOI
A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66T Encoded in a Sizeable Polysaccharide Utilization Locus.
Mikkel Schultz-Johansen,Pernille Kjersgaard Bech,Rosanna C. Hennessy,Mikkel A. Glaring,Tristan Barbeyron,Mirjam Czjzek,Peter Stougaard +6 more
TL;DR: This study reports for the first time, the discovery of novel enzyme activities targeting furcellaran, a hybrid carrageenan containing both β-carrageenan and κ/β-c Carrageenans motifs, which represent a new subfamily within the CAZy classification.
Journal ArticleDOI
Host-derived population genomics data provides insights into bacterial and diatom composition of the killer whale skin.
Rebecca Hooper,Jaelle C. Brealey,Tom van der Valk,Antton Alberdi,John W. Durban,Holly Fearnbach,Kelly M. Robertson,Robin W. Baird,M. Bradley Hanson,Paul R. Wade,M. Thomas P. Gilbert,M. Thomas P. Gilbert,Phillip A. Morin,Jochen B. W. Wolf,Jochen B. W. Wolf,Andrew D. Foote,Katerina Guschanski +16 more
TL;DR: This work uses sequence data originally generated from killer whale Orcinus orca skin biopsies for population genomics, to characterize the skin microbiome and investigate how host social and geographical factors influence the microbial community composition.
Journal ArticleDOI
Ancient acquisition of "alginate utilization loci" by human gut microbiota.
Sophie Mathieu,Mélanie Touvrey-Loiodice,Laurent Poulet,Sophie Drouillard,Renaud Vincentelli,Bernard Henrissat,Bernard Henrissat,Bernard Henrissat,Gudmund Skjåk-Bræk,William Helbert +9 more
TL;DR: A phylogenetic analysis of family PL17 reveals that all alginate lyases, which have all the same specificity and mode of action, cluster together in a very distinct subfamily as mentioned in this paper.
Journal ArticleDOI
The Pros and Cons of Using Algal Polysaccharides as Prebiotics.
Martin Gotteland,Karla Riveros,Naschla Gasaly,Constanza Carcamo,Fabien Magne,Gianella Liabeuf,Alejandra Beattie,Sebastián Rosenfeld,Sebastián Rosenfeld +8 more
TL;DR: The results support the use of laminaran, alginate and agaro-oligosaccharides as prebiotics while more studies are necessary regarding that of fucoidan, carrageenan and ulvan.
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