Journal ArticleDOI
Lipid composition of beef heart ventricle.
Manik L. Das,George Rouser +1 more
TLDR
The lipid class composition of beef heart ventricle was determined by a combination of diethylaminoethyl cellulose column chromatography and quantitative thin-layer chromatography.Abstract:
The lipid class composition of beef heart ventricle was determined by a combination of diethylaminoethyl cellulose column chromatography and quantitative thin-layer chromatography. Percentages of the total lipid were: triglyceride, 43.6; cholesterol, 7.4; phosphatidyl choline, 22.8; phingomyelin, 4.0; phosphatidyl ethanolamine, 11.2; diphosphatidyl glycerol, 5.8; phosphatidyl serine, 1.2; phosphatidyl inositol, 3.0; phosphatidyl glycerol, 0.9; unsaturated hydrocarbon, 0.02; saturated hydrocarbon, 0.20. Nonlipid components represented 33.6% of the crude chloroform/methanol extracts.read more
Citations
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Journal ArticleDOI
The composition, structure and function of lipids in the tissues of ruminant animals.
TL;DR: The fatty acids of ruminant tissues are characterized by comparatively high concentrations of odd-chain and branched-chain fatty acids and of positional and configurational isomers of mono- and diuns saturated fatty acids, and, at the same time, by comparatively low concentrations of polyunsaturated fatty acids.
Journal ArticleDOI
The Regulation of Ketogenesis from Octanoic Acid THE ROLE OF THE TRICARBOXYLIC ACID CYCLE AND FATTY ACID SYNTHESIS
TL;DR: Surprisingly, the depression of lipogenesis accompanying fasting was found to be of sufficient magnitude to account for a major proportion of the increased C2 unit flux into ketone bodies.
Book ChapterDOI
Chapter ix – content, composition, and metabolism of mammalian and avian lipids that contain ether groups
Journal ArticleDOI
The regulation of ketogenesis from oleic acid and the influence of antiketogenic agents.
J. D. McGarry,Daniel W. Foster +1 more
TL;DR: The data obtained established that the enhanced ketogenesis characteristic of livers from fasted animals could not be accounted for by depressed Krebs cycle activity and that factors affecting both the generation of acetyl-CoA and its disposal in nonketogenic pathways play important roles.
Journal ArticleDOI
Characterization of a C-5,13-Cleaving Enzyme of 13(S)-Hydroperoxide of Linolenic Acid by Soybean Seed.
TL;DR: An activity was found in mature soybean seeds that cleaved 13(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid (13S-HPOT) into 13-oxo-9 (Z), 11(E)-tridecadienoic acids and two isomeric pentenols, 2-Z)-penten-1-ol and 1-penten
References
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Journal ArticleDOI
Lipid composition of beef brain, beef liver, and the sea anemone. Two approaches to quantitative fractionation of complex lipid mixtures.
TL;DR: Two new schemes for fractionation of complex lipid mixtures are presented and the types of lipid-nonlipid interactions disclosed by column chromatography and their potential application to biological problems are discussed.
Journal ArticleDOI
Analytical separation of nonlipid water soluble substances and gangliosides from other lipids by dextran gel column chromatography.
A. N. Siakotos,George Rouser +1 more
TL;DR: After passage through the dextran gel columns, lipids eluted from thin-layer chromatograms were found to give infrared spectra identical to those of pure samples obtained by other procedures.
Journal ArticleDOI
Quantitative chromatographic fractionation of complex lipid mixtures: Brain lipids
Journal ArticleDOI
Lipids of subcellular particles.
Sidney Fleischer,George Rouser +1 more
TL;DR: It is concluded on the basis of new data obtained from highly purified mitochondria and improved analytical methods that phosphatidyl inositol is not a major component of mitochondria, and there are two basically different types of membranous structures.
Journal ArticleDOI
Analytical fractionation of complex lipid mixtures: DEAE cellulose column chromatography combined with quantitative thin layer chromatography
TL;DR: Using diethylaminoethyl cellulose column chromatography followed by thin layer chromatography for the fractionation of complex lipid mixtures with results obtained with beef brain and beef heart mitochondrial lipids is described.
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