Journal ArticleDOI
Localization of the globin gene in the template active fraction of chromatin of Friend leukemia cells.
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TLDR
The results demonstrate the success of the fractionation procedure and the globin gene sequence appears to be in an active configuration in uninduced as well as in induced Friend leukemia cells.Abstract:
Friend leukemia cell chromatin has been fractionated into template active and inactive components. The globin gene sequence is associated with the template active component both prior to and after the cells are induced with dimethyl sulfoxide to synthesize hemoglobin and therefore appears to be in an active configuration in uninduced as well as in induced Friend leukemia cells. In cells which have lost the ability to produce hemoglobin, the globin gene sequence is not associated with the template active fraction of chromatin. These results demonstrate the success of the fractionation procedure.read more
Citations
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Journal ArticleDOI
Oxidative stress as a causal factor in differentiation and aging: a unifying hypothesis
R.S. Sohal,R.G. Allen +1 more
TL;DR: The authors suggest that the damage accumulated during aging is a secondary effect rather than a direct cause of senescence, and show that cells exert control not only on their level of antioxidant defense but also on their rate of oxidant production.
Journal ArticleDOI
Oxidative influence on development and differentiation: an overview of a free radical theory of development.
R. G. Allen,Arthur K. Balin +1 more
TL;DR: The interaction of oxidants with ion balance and cytoskeleton is discussed andabolically generated oxidants have been implicated as one factor that directs the initiation of certain developmental events.
Journal ArticleDOI
Fractionation of hen oviduct chromatin into transcriptionally active and inactive regions after selective micrococcal nuclease digestion.
Kerry Bloom,John Anderson +1 more
TL;DR: Results suggest that micrococcal nuclease selectively excises nucleosomes from oviduct chromatin containing the ovalbumin gene, which permits the recovery of mononucleosomes in the 1SF which are enriched in the oval bumin gene and oligonucleotides in the 2SFWhich are depleted in this sequence.
Book ChapterDOI
Structure of Transcribing Chromatin
TL;DR: There is a need for careful well-controlled studies and the use of antibodies directed against the various H1 variants should be very useful in elucidating whether they are associated with the isolated “active” subunits as they are with theisolated nucleosomes of bulk chromatin.
Journal ArticleDOI
Oxygen-Reactive Species and Antioxidant Responses during Development: The Metabolic Paradox of Cellular Differentiation:
TL;DR: Evidence is presented that implicates oxidants as a factor that can stimulate alterations in gene expression, and possible mechanisms by which oxidants influence gene expression are discussed.
References
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Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
Chromosomal subunits in active genes have an altered conformation
Harold Weintraub,Mark Groudine +1 more
TL;DR: The results indicate that active genes are probably associated with histones in a subunit conformation in which the associated DNA is particularly sensitive to digestion by deoxyribonuclease I.
Journal ArticleDOI
Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide.
TL;DR: This action of dimethyl sulfoxide, which was reversible, may represent the derepression of leukemic cells to permit their maturation in cloned line of murine virus-induced erythroleukemia.
Hemoglobin Synthesis inMurineVirus-Induced Leukemic Cells InVitro: Stimulation ofErythroid Differentiation by Dimethyl Sulfoxide
TL;DR: In this paper, the effect of DMSO on leukemic cells was investigated and it was shown that 2% methyl sulfoxide (DMS0) increased the synthesis of hematopoietic cells.
Book ChapterDOI
Analysis of repeating DNA sequences by reassociation
TL;DR: This chapter describes techniques for the analysis of repeating DNA sequences by reassociation and a method for the evaluation of rate constants.
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Chromosomal subunits in active genes have an altered conformation
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