LYSOSOMES IN SKELETAL MUSCLE TISSUE: Zonal Centrifugation Evidence for Multiple Cellular Sources
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Postnuclear supernates from homogenates of skeletal muscle from rats subjected to starvation and injections of Triton WR-1339 and dextran + corticosterone were fractionated, indicating the presence of two groups of lysosome-like particles from muscle cells.Abstract:
Postnuclear supernates from homogenates of skeletal muscle from rats subjected to starvation, injections of Triton WR-1339, dextran-500, and dextran + corticosterone were fractionated by means of rate and isopycnic zonal centrifugation in sucrose—0.02 M KCl gradients. Zonal fractions were analyzed for protein, RNA, cytochrome oxidase, and up to six acid hydrolases. The results indicate the presence of two groups of lysosome-like particles. One group contributes approximately 95% of the cathepsin D and acid phosphatase activity and 75% of the acid ribonuclease, β-glucuronidase, and arylsulfatase activity in muscle. It is characterized by a modal equilibrium density of 1.18 that is decreased by starvation, but is not shifted by dextran-500 or Triton WR-1339. The second group has a higher proportion of acid ribonuclease, β-glucuronidase, and arylsulftase; the equilibrium density can be shifted by dextran-500 and Triton WR-1339. It is suggested that this group of lysosomes is derived from macrophages and other connective tissue cells, whereas the former group represents lysosome-like particles from muscle cells.read more
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Lysosomes and Related Particles
TL;DR: This chapter discusses lysosomes and related particles, which are particles in which intravenously injected peroxidase is concentrated and which when isolated shortly after the injection of the protein behave very much like lyssomes.
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Zanvil A. Cohn,Edith Wiener +1 more
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