Manifestation of thrombocytopenia in dengue-2-virus-infected mice.
Kao Jean Huang,Shu-Yi J. Li,Shiour-Ching Chen,Hsiao Sheng Liu,Yee Shin Lin,Trai Ming Yeh,Ching Chuan Liu,Huan Yao Lei +7 more
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TLDR
This dengue-2-virus-infected mouse system accompanied by thrombocytopenia and anti-platelet antibody will be a valuable model to study the pathogenicity of d Dengue virus infection.Abstract:
Dengue virus infection causes dengue fever, dengue haemorrhagic fever and dengue shock syndrome. No animal model is available that mimics these clinical manifestations. In this study, the establishment is reported of a murine model for dengue virus infection that resembles the thrombocytopenia manifestation. Dengue-2 virus (dengue virus type 2) can infect murine cells either in vitro (primary cell culture) or in vivo. Viraemia detected by RT-PCR was found transiently at 2 days after intravenous injection of dengue-2 virus. Transient thrombocytopenia developed at 10-13 days after primary or secondary infection. Anti-platelet antibody was generated after dengue-2 virus infection. There was strain variation in dengue-2 virus infection; the A/J strain was more sensitive than BALB/c or B6 mice. This dengue-2-virus-infected mouse system accompanied by thrombocytopenia and anti-platelet antibody will be a valuable model to study the pathogenicity of dengue virus infection.read more
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References
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TL;DR: This work has identified a severe syndrome, dengue hemorrhagic fever/dengue shock syndrome, in Southeast Asian children, which recently has also been identified in children infected with the virus in Puerto Rico.
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Scott B. Halstead,EJ O'Rourke +1 more
TL;DR: In vitro antibody-dependent infection of PBL provides a possible model for study of pathogenetic mechanisms in infants with dengue shock syndrome who passively acquire maternal anti-dengue IgG.
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E A Henchal,J R Putnak +1 more
TL;DR: Patients present with a severe febrile illness characterized by abnormalities of hemostasis and increased vascular permeability, which in some instances results in a hypovolemic shock.
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Dengue Virus Structural Differences That Correlate with Pathogenesis
Katrin C. Leitmeyer,David W. Vaughn,Douglas M. Watts,Rosalba Salas,Iris Villalobos,de Chacon,Celso Ramos,Rebeca Rico-Hesse +7 more
TL;DR: A method for comparing dengue type 2 genomes (reverse transcriptase PCR in six fragments) directly from patient plasma is developed to better reflect the true composition of viral RNA populations in the natural host and permit their association with pathogenesis.