Journal ArticleDOI
Measurement of thymine dimers in DNA by thin-layer chromatography. II. The use of one-dimensional systems
Kem H. Cook,Erroll C. Friedberg +1 more
TLDR
One combination, viz., watersaturated ethylacetate: n -propanol on silica gel thin layers was found to be particularly useful in separating thymine dimers from thymine by one-dimensional chromatography.About:
This article is published in Analytical Biochemistry.The article was published on 1976-06-01. It has received 54 citations till now. The article focuses on the topics: Thymine & Thin-layer chromatography.read more
Citations
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Book ChapterDOI
The Induction and Repair of DNA Photodamage in the Environment
David L. Mitchell,Deneb Karentz +1 more
TL;DR: Understanding of the molecular events that determine biological effects of UVR in complex higher eukaryotes is far from complete.
Journal ArticleDOI
Photosensitized reactions of nucleic acids
TL;DR: The main effects of near-ultraviolet and visible light on cellular DNA are reviewed with emphasis on base lesions, oligonucleotide single-strand breaks and DNA-protein cross-links.
Journal ArticleDOI
A pyrimidine dimer–DNA glycosylase activity associated with the v gene product of bacteriophage T4
E. H. Radany,Errol C. Friedberg +1 more
TL;DR: Early studies from this laboratory provided evidence for a role of the v gene in the excision of pyrimidine dimers from DNA, and it was shown that extracts of T4v+-infected Esherichia coli catalyse the formation of single-strand breaks and/or alkali-labile sites in UV-irradiated duplex DNA.
Journal ArticleDOI
An action spectrum for cell killing and pyrimidine dimer formation in chinese hamster v‐79 cells
R. H. Rothman,Richard B. Setlow +1 more
TL;DR: An action spectrum based on microorganisms should be adequate for extrapolation to humans in terms of risk due to ozone depleteion and is consistent with the notion that damage to DNA is the principle cause of cell death and that the most important type of damage is the pyrimidine dimer.
Journal ArticleDOI
Ultraviolet action spectra for DNA dimer induction, lethality, and mutagenesis in Escherichia coli with emphasis on the UVB region.
TL;DR: Ultraviolet action spectra were obtained for lethality and mutagenesis (reversion to tryptophan independence) in Escherichia coli WP2s for wavelengths 254–405 nm with detailed analysis in the UVB region with precise energy distributions throughout the isolated Xe bands regions.
References
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Journal ArticleDOI
Dark Repair of Ultraviolet-Irradiated Deoxyribonucleic Acid by Bacteriophage T4: Purification and Characterization of a Dimer-Specific Phage-Induced Endonuclease
Errol C. Friedberg,John J. King +1 more
TL;DR: The purification and properties of an ultraviolet (UV) repair endonuclease are described and the specific substrate in UV-irradiated DNA appears to be pyrimidine dimer sites.
Journal ArticleDOI
Mechanism of repair of DNA in bacteriophage: I. Excision of pyrimidine dimers from ultraviolet-irradiated DNA by an extract of T4-infected cells☆
Mutsuo Sekiguchi,Seiichi Yasuda,Shunzo Okubo,Hiroaki Nakayama,Kazunori Shimada,Yasuyuki Takagi +5 more
TL;DR: It is likely that enzyme(s) responsible for excision of dimers is induced by infection with T4, and the excision in vitro is similar to the reaction observed in vivo in ultraviolet-resistant cells.
Journal ArticleDOI
The role of thymine dimer in the photo-inactivation of the bacteriophage T4υ1
TL;DR: Thymine dimer formation and ultraviolet inactivation at 260 mμ have been compared on one and the same population of the bacteriophage T4υ1 and yields the result that most thymineDimer molecules cannot be interstrand crosslinks.
Journal ArticleDOI
Measurement of thymine dimers in DNA by thin-layer chromatography.
TL;DR: An adaption of two-dimensional chromatography to thin-layer cellulose is described for the separation of thymine monomers from dimers, finding a number of specific advantages over paper chromatography.
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