Showing papers in "Journal of Molecular Biology in 1963"

TL;DR: The biological activity of many proteins is controlled by specific metabolites which do not interact directly with the substrates or products of the reactions, and it is suggested that this mechanism plays an essential role in the regulation of metabolic activity.

TL;DR: The results obtained show that the filaments are structurally polarized, and in muscle are arranged so that all of them attached on one side of a given Z-line point in one direction, whilst those on the other are oppositely oriented.

TL;DR: To determine the form of replicating DNA, E. coli B3 and K12 Hfr were labelled for various periods with [3H]thymidine and their DNA was then extracted gently and observed by autoradiography.

TL;DR: There is good evidence that each of the globular subunits seen in the electron microscope represents one actin monomer, and it is suggested that both (i) and (ii) could be due to the combination of actin with other material, possibly tropomyosin B.

TL;DR: The RNA is shown to have several unusual properties; in 0·2 M-NaCl it has a very small radius of gyration and a large sedimentation coefficient for its molecular weight, indicating a very compact structure.

TL;DR: The polyphenylalanine chain, made by poly U-directed† synthesis in the cell-free system from Escherichia coli, is studied and the bond between the polypetide chain and the S-RNA is similar to that in amino acyl S- RNA but is an order of magnitude more stable against alkaline hydrolysis or hydroxylamine treatment.

TL;DR: The results indicate that many of the aggregated ribosomes contain messenger RNA, but are not receptive to added messenger RNA; whereas many ofThe unaggregated ribsomes do not contain messengerRNA,but are receptive toadded messenger RNA molecules.

TL;DR: It is suggested that the observed reflections arise almost entirely from the A band and that their relative intensity depends on the extent of overlap between the actin and myosin filaments, in agreement with the sliding-filament model proposed to account for length changes in striated muscle.

TL;DR: Hydrolysis revealed that the compound was incorporated into the DNA as such, the replacement of thymine amounting to up to 10%.

TL;DR: Analysis of the “soluble” and ribosomal proteins by starch gel electrophoresis appears to rule out the possibility that the end groups of the soluble protein fraction are contributed by contaminating ribosome proteins.

TL;DR: An improved α-diagram has been obtained from a native molluscan “catch” muscle (the anterior byssal retractor muscle of Mytilus edulis) and indicates that the best fit is obtained with a two-chain structure when various types of disorder are assumed.

TL;DR: In this paper, the poly U-directed synthesis of polyphenylalanine in the cell-free system from Escherichia coli is used as a model system in which to investigate the interaction of messenger RNA with ribosomes.

TL;DR: By denaturation studies, it is concluded that the minor as well as the major DNA components are all two-stranded.

TL;DR: A small fraction of the S-RNA that is bound to the ribosomes in high magnesium becomes resistant to being washed off the ribsomes in low magnesium, and is resistant to ribonuclease under mild conditions.

TL;DR: Observations suggest that the soluble RNA and ribosome fractions are regulated independently, and the rate of protein synthesis per unit weight of ribosomal RNA is a constant at all growth rates.

TL;DR: The number of peptides found in the fingerprint is one-half the number calculated from the amino acid analysis and the molecular weight, indicating that alkaline phosphatase is a dimer of two identical subunits, indicating the significance of this result to genetic complementation between phosphate negative mutants is discussed.

TL;DR: A class of mutants not previously described, which includes mutants lacking galactose-permease, has been demonstrated, which can be grown on agar supplemented by sufficient concentrations of Galactose and fucose in these mutants.

TL;DR: The deoxyribonucleohistone component may be physically separated from the other components of pea embryo chromatin because of its higher melting temperature, in its histone/DNA mass ratio and in its inactivity in the support of DNA-dependent RNA synthesis.

TL;DR: A mutant carrying a deletion for a small part of the cluster of eight histidine genes in Salmonella has been studied, and a number of secondary mutants have been isolated that can grow on the biosynthetic intermediate, histidinol, as a source of histidine.

TL;DR: An X-ray diffraction study of the synthetic DNA copolymers dAT† and dA BU ¯ has demonstrated that the lithium salts exist in a B form with helix dimensions identical with those of lithium DNA.

TL;DR: Results support the theory of hybrid protein formation which has been proposed to account for intra-cistron complementation and support the normal alkaline phosphatase protein is composed of two identical subunits whose structure is determined by a single functioning genetic unit.

TL;DR: Evidence was provided that the heavy complex was formed by attachment of several ribosome particles to a single molecule of the polymer, and that the polymer was attached to ribosomes on their 30 s subunit.

TL;DR: X-ray photographs of wet apoferritin crystals are found to contain both sharp reflections, extending to spacings of 1/1·4 A −1 , and diffuse streaks indicative of a structure having “ordered disorder”.

TL;DR: Results appear to identify the O c gal mutation as a constitutive operator mutation and the Gal segment as a typical operon, fortified by the observation that the galactokinase and transferase activities are quantitatively coordinated.

TL;DR: Spectrochemical and chemical analyses established the ratios of protein, lipid, organic phosphorus, iron, manganese, copper and chlorophyll in the lamellae, supporting the view that the lameLLae consist of proteins embedded in a lipid matrix.

TL;DR: The sum of the evidence supports the current hypothesis that protein synthesis in bacteria occurs on polyribosomes, each composed of a number of ribosomes serviced by a molecule of M-RNA; the functioning complexes may be stabilized on the cell membrane.

TL;DR: Observations suggest that membrane-bound ribosomes are the major site of protein synthesis in an in vitro protein synthesizing system and incorporate labeled phenylalanine while free ribosome are virtually inert.

TL;DR: In vivo complementation has been observed between alkaline phosphatase structural mutants of E. coli, and this observation, as well as other properties of the complementing strains, suggests that phosphat enzyme complementation results from the formation of hybrid enzyme molecules.

TL;DR: When the myelin sheath of peripheral nerve is expanded in hypotonic solutions the lipoprotein layers become separated by water layers but remain structurally intact and provide information required for the calculation of the electron density distribution through the layer.