Journal ArticleDOI
Medium optimization for hen egg white lysozyme production by recombinant Aspergillus niger using statistical methods.
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TLDR
Statistics-based experimental design revealed that peptone, starch, and ammonium sulfate were the most significant factors, whereas the other factors were not important within the levels tested.Abstract:
Statistics-based experimental design was used to investigate the effect of medium components (starch, peptone, ammonium sulfate, yeast extract, and CaCl2.2H2O) on hen's egg white lysozyme production by Aspergillus niger HEWL WT-13-16. A 2(5-1) fractional factorial design augmented with center points revealed that peptone, starch, and ammonium sulfate were the most significant factors, whereas the other factors were not important within the levels tested. The method of steepest ascent was used to approach the proximity of optimum. This task was followed by a central composite design to develop a response surface for medium optimization. The optimum medium composition for lysozyme production was found to be: starch 34 g L-1, peptone 34 g L-1, ammonium sulfate 11.9 g L-1, yeast extract 0.5 g L-1, and CaCl2.2H2O 0.5 g L-1. This medium was projected to produce, theoretically, 212 mg L-1 lysozyme. Using this medium, an experimental maximum lysozyme concentration of 209+/-18 mg L-1 verified the applied methodology.read more
Citations
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Journal ArticleDOI
Bioprocess optimization using design-of-experiments methodology.
TL;DR: This review surveys recent applications of design‐of‐experiments (DoE) methodology in the development of biotechnological processes and highlights a few selected examples from antibiotic fermentation, expanded bed optimization, virus vector transfection of insect cell cultivation, feed profile adaptation, embryonic stem cell expansion protocols, and mammalian cell harvesting.
Journal ArticleDOI
Enzymatic lysis of microbial cells.
Oriana Salazar,Juan A. Asenjo +1 more
TL;DR: Some of the recent advances in specific enzyme systems for bacteria and yeast cells rupture and other applications are examined and emphasis is focused in biotechnological aspects of these enzymes.
Journal ArticleDOI
Medium optimization for the production of avermectin B1a by Streptomyces avermitilis 14-12A using response surface methodology
Hong Gao,Mei Liu,Jintao Liu,Huanqin Dai,Xianlong Zhou,Xiangyang Liu,Ying Zhuo,W.S. Zhang,Lixin Zhang +8 more
TL;DR: Response surface methodology was employed to optimize the composition of medium for the production of avermectin B1a by Streptomyces avermitilis 14-12A in shaker flask cultivation and revealed that the optimum values of the tested variables were 149.57 g/l corn starch and 8.92 g/L yeast extract.
Journal ArticleDOI
Optimization of phenol degradation by Candida tropicalis Z-04 using Plackett-Burman design and response surface methodology
TL;DR: Optimal conditions obtained in this experiment laid a solid foundation for further use of this microorganism in the treatment of high-strength phenol effluents and indicated the excellent ability of C. tropicalis Z-04 in degrading high- strength phenol.
Journal ArticleDOI
Application of statistical design for the optimization of amino acid separation by reverse-phase HPLC
TL;DR: The best levels of the five independent variables that provide the maximum modified resolution for each pair of consecutive amino acids appearing in the chromatograph were determined and are of utmost importance for accurate analysis of a subset of amino acids.
References
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Applied Statistics and Probability for Engineers
TL;DR: Montgomery and Runger's Engineering Statistics text as discussed by the authors provides a practical approach oriented to engineering as well as chemical and physical sciences by providing unique problem sets that reflect realistic situations, students learn how the material will be relevant in their careers.
Journal ArticleDOI
The induction and repression of nitrate reductase in the fungus Aspergillus nidulans.
TL;DR: The result of experiments indicate that the synthesis of nitrate reductase is induced by nitrate and repressed by ammonium, and no effect in vitro of ammonium could be found.
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Statistical Design and Analysis of Experiments, with Applications to Engineering and Science
TL;DR: In this paper, the design and analysis of fractional fractional factorial experiments with random factor levels is discussed. But the focus of this paper is not on the design of the blocks, but on the analysis of the entire block.
Journal ArticleDOI
Response surface analysis and simulation as a tool for bioprocess design and optimization
TL;DR: In this article, factorial design and response surface techniques were used in combination with modeling and simulation to design and optimize an industrial bioprocess with multiple stages, where the fermentation system is composed of four ideal continuous-stirred tank reactors (ICSTR), linked in series, with cell recycling.
Journal ArticleDOI
Xylanase production in solid state fermentation by Aspergillus niger mutant using statistical experimental designs.
TL;DR: Under the optimized conditions, the activity and productivity of xylanase obtained after 5 days of fermentation were 5,071 IU/g of rice straw and 14,790 IU l–1 h–1, respectively.