Journal ArticleDOI
Membrane-bound ATPase of intact vacuoles and tonoplasts isolated from mature plant tissue.
TLDR
Intact vacuoles were isolated from petals of Hippeastrum and Tulipa and the ATPase activity of fresh vacuole suspensions was found to be 2--3 times that of protoplasts from the same tissue.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1977-02-14. It has received 117 citations till now. The article focuses on the topics: Vacuole & ATPase.read more
Citations
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Book ChapterDOI
Senescence and Postharvest Physiology of Cut Flowers, Part 1
Abraham H. Halevy,Shimon Mayak +1 more
Journal ArticleDOI
Hydrolytic enzymes in the central vacuole of plant cells.
Thomas Boller,Hans Kende +1 more
TL;DR: The data support the hypothesis that the central vacuole of higher plant cells has an enzyme composition analogous to that of the animal lysosome.
Journal ArticleDOI
Content and vacuole extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts
TL;DR: Data are presented which indicate that vacuoles obtained by gentle osmotic shock of protoplasts in dibasic phosphate have good purity and retain their contents.
Journal ArticleDOI
ATP-Driven H+ Pumping into Intracellular Organelles
TL;DR: This review will focus on the evi dence that intracellular organelles are acidic, the mechanism by which the organelle ATPase pumps H+ ions, and the way that variousOrganelles utilize the ATP-generated H+ potential.
Journal ArticleDOI
Partition of membrane particles in aqueous two-polymer phase system and its practical use for purification of plasma membranes from plants.
TL;DR: A simplified method for the isolation of a plasma membrane-enriched fraction from plants utilizing an aqueous two-polymer phase system is outlined and may be applicable to a wide variety of plants and plant tissue including green leaves.
References
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Book ChapterDOI
Purification of a plasma membrane-bound adenosine triphosphatase from plant roots.
T.K. Hodges,R.T. Leonard +1 more
TL;DR: Using continuous gradient centrifugation and taking advantage of differing pH and ion-sensitivities of the various ATPases, the chapter has distinguished 5 different membrane-associated ATPases in oat root extracts.
Journal ArticleDOI
Isolation and Properties of the Envelope of Spinach Chloroplasts
TL;DR: The method makes use of the fact that gentle swelling of intact chloroplasts causes breakage and total detachment of the envelope to isolate separately the envelope and thylakoids of chloroplast.
Journal ArticleDOI
Partial Resolution of the Enzymes Catalyzing Oxidative Phosphorylation XIII. STRUCTURE AND FUNCTION OF SUBMITOCHONDRIAL PARTICLES COMPLETELY RESOLVED WITH RESPECT TO COUPLING FACTOR 1
TL;DR: Reconstituted SU-particles catalyzed oxidative phosphorylation with a P:O ratio of 0.5 and were morphologically indistinguishable from untreated submitochondrial particles, indicating that coupling factor 1 is responsible for most, if not all, of the inner membrane spheres.
Journal ArticleDOI
Partial Resolution of the Enzymes Catalyzing Photophosphorylation: II. THE INHIBITION AND STIMULATION OF PHOTOPHOSPHORYLATION BY N,N'-DICYCLOHEXYLCARBODIIMIDE
Richard E. McCarty,Efraim Racker +1 more
TL;DR: Dicyclohexylcarbodiimide inhibited the pH decay, indicating that its stimulation of the light-induced pH rise might be due to an inhibition of the breakdown of a high energy intermediate in chloroplasts.