Metabolism of Prostaglandin E2 in Guinea Pig Liver I. IDENTIFICATION OF SEVEN METABOLITES
Mats Hamberg,Ulf Israelsson +1 more
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TLDR
Seven metabolites were isolated by reversed phase partition chromatography and thin layer chromatography after incubation of tritium-labeled prostaglandin E2 with the soluble fraction of homogenates of guinea pig liver and showed that prostaglandsin Fα compounds can be formed from prostag landin E compounds in animal tissue.About:
This article is published in Journal of Biological Chemistry.The article was published on 1970-10-10 and is currently open access. It has received 99 citations till now. The article focuses on the topics: Prostaglandin & Prostaglandin E.read more
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Isolation and Structure of Two Prostaglandin Endoperoxides That Cause Platelet Aggregation
TL;DR: Platelet aggregation induced by thrombin was accompanied by release of material reducible by stannous chloride into prostaglandin F(2alpha), thus indicating the involvement of endogenous prostaglandsin endoperoxides in platelet aggregation.
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On the Metabolism of Prostaglandins E1 and E2 in Man
Mats Hamberg,Bengt Samuelsson +1 more
TL;DR: 7α-Hydroxy-5,11-diketotetranor-prosta-1,16-dioic acid was identified as the major urinary metabolite after intravenous administration of tritium-labeled prostaglandin E2 and was rapidly converted initially into 11α-hydroxy-9, 15- diketoprost-5-enoic acid.
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On the mechanism of prostacyclin and thromboxane A2 biosynthesis.
Markus Hecker,Volker Ullrich +1 more
TL;DR: A cage radical mechanism is proposed for both enzymes according to which the initial radical process is terminated through oxidation of carbon-centered radicals by the iron-sulfur catalytic site, followed by ionic rearrangement to PGI2 or TXA2.
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Human Microsomal Prostaglandin E Synthase-1 PURIFICATION, FUNCTIONAL CHARACTERIZATION, AND PROJECTION STRUCTURE DETERMINATION
Staffan Thorén,Rolf Weinander,Sipra Saha,Caroline Jegerschöld,Pär L. Pettersson,Bengt Samuelsson,Hans Hebert,Mats Hamberg,Ralf Morgenstern,Per-Johan Jakobsson +9 more
TL;DR: Human, microsomal, and glutathione-dependent prostaglandin (PG) E synthase-1 was expressed with a histidine tag in Escherichia coli and it was demonstrated thatMicrosomal PGES-1 constitutes a trimer in the crystal, i.e. an organization similar to the microsomeglathione transferase 1.
References
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Prostaglandins and related factors: xix. thin-layer chromatography of prostaglandins.
Krister Gréen,Bengt Samuelsson +1 more
TL;DR: In this paper, the authors described methods for separation of all known prostaglandins and some derivatives as free acids and methyl esters by thin-layer chromatography both on analytical and preparative scales.
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On the Mechanism of the Biosynthesis of Prostaglandins E1 and F1α
Mats Hamberg,Bengt Samuelsson +1 more
TL;DR: It is suggested that 11-peroxy-8,12,14-eicosatrienoic acid is the first intermediate in the conversion, which is eventually transformed into PGE1 or PGF1α by independent reactions.
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Prostaglandins in Human Seminal Plasma PROSTAGLANDINS AND RELATED FACTORS 46
Mats Hamberg,Bengt Samuelsson +1 more
TL;DR: This study on human seminal plasma sought after the compounds which either possess the dienone chromophore or can be converted into it by treatment with sodium hydroxide and led to the isolation of 8 more prostaglandins which were present in higher concentrations than the previously recognized PGs.
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Prostaglandins and related factors. 28. metabolism of prostaglandin e1 in guinea pig lung: the structures of two metabolites.
Journal ArticleDOI
The enzymic conversion of all‐cis 8,11,14‐eicosatrienoic acid into prostaglandin E1
TL;DR: A scheme for the mechanism of the conversion is proposed in which the formation of PGE1 as well as that of the byproducts is assumed to occur on the enzyme in concerted reactions via peroxy-radicals and a cyclic peroxide as mentioned in this paper.