Journal ArticleDOI
Metabolome classification of commercial Hypericum perforatum (St. John's Wort) preparations via UPLC-qTOF-MS and chemometrics.
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TLDR
This study provides the first approach utilizing UPLC-MS-based metabolic fingerprinting to reveal secondary metabolite compositional differences in Hypericum extract, and is believed to be the first in the world to do so.Abstract:
The growing interest in the efficacy of phytomed- icines and herbal supplements but also the in- crease in legal requirements for safety and reli- able contents of active principles drive the devel- opment of analytical methods for the quality con- trol of complex, multicomponent mixtures as found in plant extracts of value for the pharma- ceutical industry. Here, we describe an ultra-per- formance liquid chromatography method (UPLC) coupledwith quadrupole time of flight massspec- trometry (qTOF‑MS) measurements for the large scale analysis of H. perforatum plant material and its commercial preparations. Under optimized conditions, we were able to simultaneously quan- tify and identify 21 metabolites including 4 hy- perforins, 3 catechins, 3 naphthodianthrones, 5 flavonoids, 3 fatty acids, and a phenolic acid. Prin- cipal component analysis (PCA) was used to en- sure good analytical rigorousness and define both similarities and differences among Hypericum samples. A selection of batches from 9 commer- cially available H. perforatum products available on the German and Egyptian marketsshowed var- iable quality, particularly in hyperforins and fatty acid content. PCA analysis was able to discrimi- nate between various preparations according to their global composition, including differentia- tion between various batches from the same sup- plier. To the best of our knowledge, this study pro- vides the first approach utilizing UPLC‑MS-based metabolic fingerprinting to reveal secondary me- tabolite compositional differences in Hypericum extract. " Hypericum perforatum L. l " Hypericaceae l " UPLC‑MS l " hyperforin l " principal component analysis l " quality control l " St. Johnʼs wortread more
Citations
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St John's wort (Hypericum perforatum L.): a review of its chemistry, pharmacology and clinical properties
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