Book ChapterDOI
Method to Study Dynamics of Membrane-Bound Plant Transcription Factors During Biotic Interactions in Tomato.
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TLDR
An Agrobacterium-based transient transformation method is reported to assay the localization of fluorescent protein-tagged MTFs in tomato leaf epidermal peels that are subsequently infected with a pathogenic fungus and facilitates the realistic monitoring of MTF movement during activation of a signaling event.Abstract:
Sequestration of a transcription factor in a cellular membrane and releasing it on demand is an additional layer of gene regulation that is considered a rapid mode to reprogram a gene expression cascade when a plasma membrane stress signal is perceived. Better understanding of the dynamic exchange of membrane-bound transcription factors (MTFs) during biotic stress requires the development of a simple, efficient, and quick assay system. Here we report an Agrobacterium-based transient transformation method to assay the localization of fluorescent protein-tagged MTFs in tomato leaf epidermal peels that are subsequently infected with a pathogenic fungus. Essentially, our method mimics natural infection and facilitates the realistic monitoring of MTF movement during activation of a signaling event.read more
References
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Journal ArticleDOI
Plant-mPLoc: A Top-Down Strategy to Augment the Power for Predicting Plant Protein Subcellular Localization
Kuo-Chen Chou,Hong-Bin Shen +1 more
TL;DR: A new predictor called “Plant-mPLoc” is developed by integrating the gene ontology information, functional domain information, and sequential evolutionary information through three different modes of pseudo amino acid composition that has the capacity to deal with multiple-location proteins beyond the reach of any existing predictors specialized for identifying plant protein subcellular localization.
Journal ArticleDOI
SAAT: sonication-assisted Agrobacterium-mediated transformation
Harold N. Trick,John J. Finer +1 more
TL;DR: SAAT increases transient transformation efficiency in several different plant tissues including leaf tissue, immature cotyledons, somatic and zygotic embryos, roots, stems, shoot apices, embryogenic suspension cells and whole seedlings.
Journal ArticleDOI
Exploring membrane-associated NAC transcription factors in Arabidopsis: implications for membrane biology in genome regulation
Sun-Young Kim,Sang-Gyu Kim,Youn-Sung Kim,Pil Joon Seo,Mikyoung Bae,Hye-Kyung Yoon,Chung-Mo Park +6 more
TL;DR: It is proposed that proteolytic activation of MTFs is a genome-wide mechanism regulating plant genomes and the presence of numerous MTFS is predicted in the whole transcription factors in plants.
Journal ArticleDOI
Combining Experimental and Predicted Datasets for Determination of the Subcellular Location of Proteins in Arabidopsis
TL;DR: The subcellular location database for Arabidopsis proteins (SUBA) is merged, providing an integrated understanding of protein location, encompassing the plastid, mitochondrion, peroxisome, nucleus, plasma membrane, endoplasmic reticulum, vacuole, Golgi, cytoskeleton structures, and cytosol ( www.suba.uwa.edu.au).
Journal ArticleDOI
Ultrasound: mechanical gene transfer into plant cells by sonoporation.
Yiyao Liu,H. Yang,Akio Sakanishi +2 more
TL;DR: The use of ultrasound for nonviral gene delivery has been applied for mammalian systems, which provides a fundamental basis and strong promise for development of new gene therapy methods for clinical medicine.