Journal ArticleDOI
Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge
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TLDR
Phylogenetic analysis indicated that only Clusters I and III NifH were present, consistent with the phylogenetic analysis of the microbial 16S rRNA genes, indicating that Bacteria play the main role in nitrogen fixation in this hydrothermal vent environment.Abstract:
A sediment sample was collected from a deep-sea hydrothermal vent field located at a depth of 2 951 m on the Southwest Indian Ridge. Phylogenetic analyses were performed on the prokaryotic community using polymerase chain reaction (PCR) amplification of the 16S rRNA and nifH genes. Within the Archaea, the dominant clones were from marine benthic group E (MBGE) and marine group I (MGI) belonging to the phyla Euryarchaeota and Thaumarchaeota, respectively. More than half of the bacterial clones belonged to the Proteobacteria, and most fell within the Gammaproteobacteria. No epsilonproteobacterial sequence was observed. Additional phyla were detected including the Actinobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, Nitrospirae, Chloroflexi, Chlorobi, Chlamydiae, Verrucomicrobia, and candidate divisions OD1, OP11, WS3 and TM6, confirming their existence in hydrothermal vent environments. The detection of nifH gene suggests that biological nitrogen fixation may occur in the hydrothermal vent field of the Southwest Indian Ridge. Phylogenetic analysis indicated that only Clusters I and III NifH were present. This is consistent with the phylogenetic analysis of the microbial 16S rRNA genes, indicating that Bacteria play the main role in nitrogen fixation in this hydrothermal vent environment.read more
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Journal ArticleDOI
Processes and Microorganisms Involved in the Marine Nitrogen Cycle: Knowledge and Gaps
Silvia Pajares,Ramiro Ramos +1 more
TL;DR: In this paper, the current understanding of the marine microbial N cycle, the functional N genes involved, the ecology and distribution of the participating microorganisms, and the main impacts of anthropogenic activities on the marine N cycle are summarized.
Journal ArticleDOI
Microorganisms from deep-sea hydrothermal vents
TL;DR: The taxonomic and physiological diversity of microbial prokaryotic life from cosmopolitan to endemic taxa are reviewed and emphasized and their significant roles in the biogeochemical processes in deep-sea hydrothermal vents are emphasized.
Journal ArticleDOI
Comparative metagenomic analysis of the microbial communities in the surroundings of Iheya north and Iheya ridge hydrothermal fields reveals insights into the survival strategy of microorganisms in deep-sea environments
Hai-liang Wang,Li Sun +1 more
TL;DR: The finding that genes involved in DNA repair, reductive acetyl-CoA pathway, and ammonia metabolism were possibly affected by distance to the vent fields facilitate the understanding of the strategies of the microbial communities to adapt to the environments in deep sea areas associated with hydrothermal vents.
Journal ArticleDOI
A comparative study revealed first insights into the diversity and metabolisms of the microbial communities in the sediments of Pacmanus and Desmos hydrothermal fields.
TL;DR: It was found that Gammaproteobacteria and Thaumarchaeota were the most abundant bacterial and archaeal populations, respectively in Pacmanus and Desmos hydrothermal fields, and Illumina-based sequencing of 16S rRNA gene amplicons and metagenomic analysis revealed new insights into the operational mechanism.
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Patrick D. Schloss,Patrick D. Schloss,Sarah L. Westcott,Sarah L. Westcott,Thomas Ryabin,Justine R. Hall,Martin Hartmann,Emily B. Hollister,Ryan A. Lesniewski,Brian B. Oakley,Donovan H. Parks,Courtney J. Robinson,Jason W. Sahl,Blaz Stres,Gerhard G. Thallinger,David J. Van Horn,Carolyn F. Weber +16 more
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