Journal ArticleDOI
Microbial utilisation of two proteins adsorbed to a vertisol clay fraction: toxin from Bacillus thuringiensis subsp. tenebrionis and bovine serum albumin
Tiphaine Chevallier,Tiphaine Chevallier,Pardon Muchaonyerwa,Pardon Muchaonyerwa,Claire Chenu +4 more
Reads0
Chats0
TLDR
The inhibitory effect of clays on utilisation of BSA and Btt toxin was interpreted as being the result of the adsorption of the proteins to clay, which rendered the proteins unavailable for microbial utilisation.Abstract:
Clay minerals have been shown to reduce the extent and rate of biodegradation of several compounds. Here, we investigated the ability of soil clays to protect proteins from biodegradation: the insecticidal protein from Bacillus thuringiensis subsp. tenebrionis ( Btt toxin) and Bovine Serum Albumin (BSA). The two proteins adsorbed in large amounts (up to 0.24 g BSA g −1 clay and 0.74 g Btt toxin g −1 clay) and irreversibly to smectite clay particles from a vertisol. We measured the growth of a soil inoculum in the presence of each of proteins as the sole source of carbon. When clay was present in the medium, microbial growth was directly proportional to the amount of free protein (i.e. nonadsorbed). Hence, the two proteins were unavailable when adsorbed to clay. The clay had little influence on the ability of microorganisms to hydrolyse a soluble substrate. The inhibitory effect of clays on utilisation of BSA and Btt toxin was interpreted as being the result of the adsorption of the proteins to clay, which rendered the proteins unavailable for microbial utilisation.read more
Citations
More filters
Journal ArticleDOI
A conceptual model of organo-mineral interactions in soils: self-assembly of organic molecular fragments into zonal structures on mineral surfaces
TL;DR: In this paper, the authors propose a structure for organo-mineral associations in soils based on recent insights concerning the molecular structure of soil organic matter (SOM), and on extensive published evidence from empirical studies of organomineral interfaces.
Journal ArticleDOI
Organic C and N stabilization in a forest soil: Evidence from sequential density fractionation
Phillip Sollins,Christopher W. Swanston,Markus Kleber,Timothy R. Filley,Marc G. Kramer,Susan E. Crow,Bruce A. Caldwell,Kate Lajtha,Richard D. Bowden +8 more
TL;DR: In this paper, an Oregon andic soil was sequentially density fractionated at 1.65, 1.85, 2.28, and 2.55 cm −3 and analyzed the six fractions for measures of organic matter and mineral phase properties.
Journal ArticleDOI
Activities of extracellular enzymes in physically isolated fractions of restored grassland soils
TL;DR: In this paper, the authors used physical fractionation procedures to investigate whether soil carbon is spatially isolated from degradative enzymes across a prairie restoration chronosequence in Illinois, USA.
Organic C and N stabilization in a forest soil: Evidence from sequential density fractionation
Phillip Sollins,Christopher W. Swanston,Markus Kleber,Timothy R. Filley,Marc G. Kramer,Susan E. Crow,Bruce A. Caldwell,Kate Lajtha,Richard D. Bowden +8 more
TL;DR: In this paper, an Oregon andic soil at 1.65, 1.85, 2.28, and 2.55 g cm was sequentially density fractionated and the six fractions were analyzed for measures of organic matter and mineral phase properties.
Journal ArticleDOI
Soil minerals and humic acids alter enzyme stability: implications for ecosystem processes
TL;DR: In this paper, rates of enzyme turnover in a volcanic soil with and without added enzymes were determined, and it was shown that adding allophane had a strong positive effect on most enzyme activities.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A general treatment and classification of the solute adsorption isotherm part. II. Experimental interpretation
TL;DR: In this article, the S-curve can be produced by either cooperative adsorption or by a competitive effect of an impurity in the solvent, which is illustrated and discussed.
Book
Interactions of soil minerals with natural organics and microbes
Pan Ming Huang,Morris Schnitzer +1 more
TL;DR: In this paper, the interactions of soil minerals with natural organics and microbes are discussed, including the interaction of soil mineral with natural organic and microbial organisms, in the context of soil mining.
Related Papers (5)
Persistence and biological activity in soil of the insecticidal proteins from Bacillus thuringiensis, especially from transgenic plants
Persistence of the insecticidal toxin from Bacillus thuringiensis subsp. kurstaki in soil
Hazel Tapp,Guenther Stotzky +1 more