Journal ArticleDOI
Microfluidic electrocapture interfaced with electrospray mass spectrometry
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TLDR
The use of electrocapture has already been demonstrated for sample clean-up, pre-concentration, chemical modification and peptide separation, all without the need for supporting gels or chemical binding as discussed by the authors.About:
This article is published in International Journal of Mass Spectrometry.The article was published on 2007-01-01. It has received 9 citations till now. The article focuses on the topics: Protein mass spectrometry & Sample preparation in mass spectrometry.read more
Citations
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Journal ArticleDOI
Bioanalytical separations using electric field gradient techniques.
TL;DR: The emerging area of electric field gradient techniques is reviewed, dividing the large variety of techniques by the target of separation, using dielectrophoresis, electric fieldgradient focusing, and focusing with centrifugal force.
Journal ArticleDOI
Cholera toxin subunit B detection in microfluidic devices
TL;DR: It was found that, in addition to a lower LoD, the electrochemical format assay showed advantages over the fluorescence format in terms of flexibility and reliability of signal recording.
Journal ArticleDOI
Functional electrospray emitters
Michel Prudent,Hubert H. Girault +1 more
TL;DR: The present review does not intend to focus on the electrospray ionisation technique in itself but rather on the introduction of functional emitters for ESI-MS and the different options available at the three different levels are reviewed to highlight the potential of functional ESI in the growing field of mass spectrometry.
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New developments in protein structure-function analysis by MS and use of hydrogen-deuterium exchange microfluidics.
TL;DR: It is demonstrated how a novel hydrogen–deuterium exchange microfluidics tool can be used online with an ESI mass spectrometer to monitor regional accessibility in a peptide, as exemplified with amyloid‐β peptide 1–40.
Journal ArticleDOI
Separation of Polypeptides by Isoelectric Point Focusing in Electrospray-Friendly Solution Using a Multiple-Junction Capillary Fractionator
Konstantin Chingin,Juan Astorga-Wells,Mohammad Pirmoradian Najafabadi,Thorleif Lavold,Roman A. Zubarev,Roman A. Zubarev +5 more
TL;DR: An online multiple-junction capillary isoelectric focusing fractionator (OMJ-CIEF) for separation of biological molecules in solution by pI has a potential of becoming a practical add-on unit in a wide range of bioanalytical setups, in particular as a first-dimension separation in mass spectrometry based proteomics.
References
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Journal ArticleDOI
Physics and applications of microfluidics in biology.
TL;DR: The focus of this review is microscale phenomena and the use of the physics of the scale to create devices and systems that provide functionality useful to the life sciences.
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Lab-on-a-chip for drug development.
TL;DR: Advances in micro-device technology for proteomics, sample preconditioning, immunoassays, electrospray ionization mass spectrometry, and polymerase chain reaction are reviewed.
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Microfluidic systems in proteomics.
Niels Lion,Tatiana C. Rohner,Loïc Dayon,Isabelle L. Arnaud,Eugen Damoc,Nikolay Youhnovski,Zhiyong Wu,Christophe Roussel,Jacques Josserand,Henrik Jeldtoft Jensen,Joël S. Rossier,Michael Przybylski,Hubert H. Girault +12 more
TL;DR: The state‐of‐the‐art in miniaturized sample preparation, immunoassays, one‐dimensional and multidimensional analyte separations, and coupling of microdevices with electrospray ionization‐mass spectrometry is presented.
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Microfabricated devices: A new sample introduction approach to mass spectrometry.
TL;DR: This review aims at documenting the technologies and applications of microfluidic mass spectrometry for the analysis of proteomic samples.