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Journal ArticleDOI

Microscopic and Spectroscopic Evaluation of Inactivation of Staphylococcus aureus by Pulsed UV Light and Infrared Heating

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TLDR
In this article, a 5-s treatment with pulsed UV light resulted in complete inactivation of Staphylococcus aureus even after enrichment, which suggested a nonthermal treatment.
Abstract
Pulsed UV light and infrared heat-treated Staphylococcus aureus cells were analyzed using transmission electron microscopy to identify the cell damage due to the treatment process. A 5-s treatment with pulsed UV light resulted in complete inactivation of S. aureus even after enrichment. The temperature increase during the pulsed UV light treatment was insignificant, which suggested a nonthermal treatment. S. aureus was also infrared heat treated using an infrared heating system with six infrared lamps. Five milliliters of S. aureus cells in phosphate buffer was treated at 700°C lamp temperature for 20 min. The microscopic observation clearly indicated that there was cell wall damage, cytoplasmic membrane shrinkage, cellular content leakage, and mesosome disintegration after both pulsed UV light and infrared treatments. Fourier transform infrared microspectrometry was successfully used to classify the pulsed UV light and infrared heat-treated S. aureus by discriminant analysis.

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Application of ultraviolet light-emitting diodes (UV-LEDs) for water disinfection: A review

TL;DR: A comprehensive review of recent studies on UV-LEDs with various wavelengths for the inactivation of different microorganisms shows many inconsistent and incomparable data were found from published studies, which underscores the importance of establishing a standard protocol for studying UV- LED in activation of microorganisms.
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Pulsed Light Treatments for Food Preservation. A Review

TL;DR: Pulsed light (PL) is an emerging nonthermal technology for decontamination of food surfaces and food packages, consisting of short time high-peak pulses of broad spectrum white light.
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Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

TL;DR: Vibrational spectroscopy, unlike other techniques used in microbiology, is a relatively simple method for studying structural changes occurring within a microbial cell following environmental stress and applications of food processing treatments.
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Study of the inactivation of spoilage microorganisms in apple juice by pulsed light and ultrasound

TL;DR: In natural apple Juice, the combination of US + 60 s PL at the highest temperature build-up was the most effective treatment for both strains, and in commercial apple juice, US did not contribute to further inactivation of spores, but significantly reduced yeast population.
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Emerging chemical and physical disinfection technologies of fruits and vegetables: a comprehensive review

TL;DR: This review comprehensively examines the working principles and applications of several emerging disinfection technologies and identifies further research opportunities for the cost-effective commercialization of these technologies.
References
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Journal ArticleDOI

Food-related illness and death in the United States.

TL;DR: Overall, foodborne diseases appear to cause more illnesses but fewer deaths than previously estimated.
Book

Infrared and Raman Characteristic Group Frequencies: Tables and Charts

TL;DR: This new edition of this highly successful manual is not only a revised text but has been extended to meet the interpretive needs of Raman users as well as those working in the IR region, creating a uniquely practical, comprehensive and detailed source for spectral interpretation.
Journal ArticleDOI

Regularized Discriminant Analysis

TL;DR: Alternatives to the usual maximum likelihood estimates for the covariance matrices are proposed, characterized by two parameters, the values of which are customized to individual situations by jointly minimizing a sample-based estimate of future misclassification risk.
Journal ArticleDOI

Improvements on Cross-Validation: The 632+ Bootstrap Method

TL;DR: It is shown that a particular bootstrap method, the .632+ rule, substantially outperforms cross-validation in a catalog of 24 simulation experiments and also considers estimating the variability of an error rate estimate.
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