Modification of microfluidic paper-based devices with silica nanoparticles
Elizabeth Evans,Ellen Flávia Moreira Gabriel,Ellen Flávia Moreira Gabriel,Tomás Enrique Benavidez,Wendell K. T. Coltro,Carlos D. Garcia +5 more
TLDR
The potential of silica nanoparticles to avoid the washing away effect and improve the color uniformity and intensity in colorimetric bioassays performed on μPADs is demonstrated.Abstract:
This paper describes a silica nanoparticle-modified microfluidic paper-based analytical device (μPAD) with improved color intensity and uniformity for three different enzymatic reactions with clinical relevance (lactate, glucose, and glutamate). The μPADs were produced on a Whatman grade 1 filter paper and using a CO2 laser engraver. Silica nanoparticles modified with 3-aminopropyltriethoxysilane were then added to the paper devices to facilitate the adsorption of selected enzymes and prevent the washing away effect that creates color gradients in the colorimetric measurements. According to the results herein described, the addition of silica nanoparticles yielded significant improvements in color intensity and uniformity. The resulting μPADs allowed for the detection of the three analytes in clinically relevant concentration ranges with limits of detection (LODs) of 0.63 mM, 0.50 mM, and 0.25 mM for lactate, glucose, and glutamate, respectively. An example of an analytical application has been demonstrated for the semi-quantitative detection of all three analytes in artificial urine. The results demonstrate the potential of silica nanoparticles to avoid the washing away effect and improve the color uniformity and intensity in colorimetric bioassays performed on μPADs.read more
Citations
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Disposable Sensors in Diagnostics, Food, and Environmental Monitoring.
Can Dincer,Richard Bruch,Estefanía Costa-Rama,M.T. Fernández-Abedul,Arben Merkoçi,Andreas Manz,Gerald Urban,Firat Güder +7 more
TL;DR: A brief insight into the materials and basics of sensors (methods of transduction, molecular recognition, and amplification) is provided followed by a comprehensive and critical overview of the disposable sensors currently used for medical diagnostics, food, and environmental analysis.
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Toward practical application of paper-based microfluidics for medical diagnostics: state-of-the-art and challenges.
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Technical aspects and challenges of colorimetric detection with microfluidic paper-based analytical devices (μPADs) - A review.
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Protein adsorption onto nanomaterials for the development of biosensors and analytical devices: A review
TL;DR: The general trend amongst the research papers allows concluding that the use of nanomaterials has already provided significant improvements in the analytical performance of many biosensors and that this research field will continue to grow.
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Paper-based analytical devices for clinical diagnosis: recent advances in the fabrication techniques and sensing mechanisms.
TL;DR: This review describes currently developed cellulose and flexible transparency paper-based microfluidic devices, device fabrication techniques, and sensing technologies that are integrated with these devices and their potential in clinical settings.
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