Journal ArticleDOI
Molecular analysis of a resistance-breaking strain of potato virus X.
Tony A. Kavanagh,Matthew G. Goulden,Simon Santa Cruz,Sean Chapman,I. Barker,David C. Baulcombe +5 more
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TLDR
Full-length cDNA clones of potato virus X (PVX) strains PVXUK3 and PVXHB have been constructed in plasmid vectors to allow in vitro transcription of infectious PVX RNA, and the coat protein gene is therefore the determinant of Nx and Rx resistance breaking of PVX HB.About:
This article is published in Virology.The article was published on 1992-08-01. It has received 124 citations till now. The article focuses on the topics: Potato virus X & Potexvirus.read more
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An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus.
TL;DR: A system based on co-expression of a viral-encoded suppressor of gene silencing, the p19 protein of tomato bushy stunt virus, that prevents the onset of PTGS in the infiltrated tissues and allows high level of transient expression is described.
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Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana.
TL;DR: By expressing PVY and CMV‐encoded proteins in a PVX vector, it is shown that the viral suppressors of gene silencing are the HCPro of PVy and the 2b protein of CMV.
Journal ArticleDOI
Initiation and Maintenance of Virus-Induced Gene Silencing
TL;DR: A model of virus-induced gene silencing (VIGS) is proposed in which the initiation of VIGS is dependent on the virus and maintenance of it is virus independent, and the VIGs of the GFP was targeted against PVX–GFP.
Journal ArticleDOI
Plant viral synergism: the potyviral genome encodes a broad-range pathogenicity enhancer that transactivates replication of heterologous viruses.
TL;DR: The results indicate that the potyviral P1/HC-Pro region affects a step in disease development that is common to a broad range of virus infections and suggest a mechanism involving transactivation of viral replication.
Journal ArticleDOI
Gene silencing without DNA. rna-mediated cross-protection between viruses
TL;DR: Analysis of plants infected with viruses that are quite distinct from the nepovirus or caulimovirus groups demonstrates that this RNA-mediated defense is a general response to virus infection, and provides direct evidence that post-transcriptional gene silencing of nuclear genes is a manifestation of a natural defense mechanism that is induced by a wide range of viruses.
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Journal ArticleDOI
A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions
TL;DR: These procedures, which can circumvent the need for large-scale phage or plasmid growths, preparative gel-electrophoresis and the screening of molecular clones, can facilitate the rapid study of sequence-specific interactions of proteins and DNA.
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Gene-for-gene complementarity in plant-pathogen interactions.
TL;DR: The cloning of avirulence genes has greatly aided the understanding of plant-pathogen specificity and established firm genetic and biochemical evidence supporting the elicitor-receptor model for recognition of incompatible pathogen races by plants.
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Oligonucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template
Mark Zoller,Michael Smith +1 more
TL;DR: A heptadecanucleotide was used to create a T----G transversion in the MATa gene of Saccharomyces cerevisiae cloned into the vector M13mp5, and the efficiency of mutagenesis was approximately 50%.
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Genetical studies on resistance to Potato viruses X and Y.
TL;DR: The present investigations concern resistance (immunity) to potato virus X and/or potato virus Y and necrotic hypersensitivity, a basis of resistance to these viruses, and there is a consensus of evidence that each form of response is under single gene control.